Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HZT22_RS16515 Genome accession   NZ_CP058833
Coordinates   3401103..3401639 (+) Length   178 a.a.
NCBI ID   WP_000168305.1    Uniprot ID   A0A9P2PPK2
Organism   Shigella flexneri strain STLEFF_27     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3375873..3421953 3401103..3401639 within 0


Gene organization within MGE regions


Location: 3375873..3421953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HZT22_RS16400 (HZT22_16320) - 3377030..3378484 (+) 1455 Protein_3210 SopA family protein -
  HZT22_RS16405 (HZT22_16325) ubiC 3378707..3379204 (+) 498 WP_001326644.1 chorismate lyase -
  HZT22_RS16410 (HZT22_16330) ubiA 3379217..3380089 (+) 873 WP_000455227.1 4-hydroxybenzoate octaprenyltransferase -
  HZT22_RS16415 (HZT22_16335) plsB 3380244..3382667 (-) 2424 WP_000017354.1 glycerol-3-phosphate 1-O-acyltransferase PlsB -
  HZT22_RS16420 (HZT22_16340) dgkA 3382838..3383206 (+) 369 WP_000002907.1 diacylglycerol kinase -
  HZT22_RS16425 (HZT22_16345) lexA 3383316..3383924 (+) 609 WP_000646078.1 transcriptional repressor LexA -
  HZT22_RS16430 (HZT22_16350) dinF 3383997..3385322 (+) 1326 WP_001326646.1 MATE family efflux transporter DinF -
  HZT22_RS16435 (HZT22_16355) - 3385438..3385647 (+) 210 WP_001030593.1 CsbD family protein -
  HZT22_RS16440 (HZT22_16360) zur 3385689..3386204 (-) 516 WP_001295691.1 zinc uptake transcriptional repressor Zur -
  HZT22_RS24505 yjbL 3386522..3386776 (+) 255 WP_000912571.1 protein YjbL -
  HZT22_RS16445 (HZT22_16365) - 3386800..3387507 (+) 708 WP_001311303.1 DUF2713 domain-containing protein -
  HZT22_RS16450 (HZT22_16370) dusA 3387870..3388907 (+) 1038 WP_001298868.1 tRNA dihydrouridine(20/20a) synthase DusA -
  HZT22_RS16455 (HZT22_16375) pspG 3389041..3389283 (+) 243 WP_000891404.1 envelope stress response protein PspG -
  HZT22_RS16460 (HZT22_16380) - 3389449..3390432 (-) 984 WP_000235508.1 quinone oxidoreductase -
  HZT22_RS16465 (HZT22_16385) dnaB 3390515..3391930 (+) 1416 WP_000918363.1 replicative DNA helicase -
  HZT22_RS16470 (HZT22_16390) alr 3391983..3393062 (+) 1080 WP_001147328.1 alanine racemase -
  HZT22_RS16475 (HZT22_16395) tyrB 3393315..3394508 (+) 1194 WP_000486985.1 aromatic amino acid transaminase -
  HZT22_RS16480 (HZT22_16400) - 3394730..3394950 (-) 221 Protein_3227 hypothetical protein -
  HZT22_RS16485 (HZT22_16405) - 3395006..3395703 (+) 698 WP_103215986.1 IS1-like element IS1A family transposase -
  HZT22_RS16490 (HZT22_16410) - 3395787..3395990 (-) 204 WP_001321562.1 protein YjbS -
  HZT22_RS16495 (HZT22_16415) aphA 3396392..3397105 (+) 714 WP_001226928.1 acid phosphatase AphA -
  HZT22_RS16500 (HZT22_16420) - 3397216..3397632 (+) 417 WP_000270375.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  HZT22_RS16505 (HZT22_16425) - 3397636..3397992 (+) 357 WP_000155657.1 MmcQ/YjbR family DNA-binding protein -
  HZT22_RS16510 (HZT22_16430) uvrA 3398027..3400849 (-) 2823 WP_000357740.1 excinuclease ABC subunit UvrA -
  HZT22_RS16515 (HZT22_16435) ssb 3401103..3401639 (+) 537 WP_000168305.1 single-stranded DNA-binding protein SSB1 Machinery gene
  HZT22_RS16520 (HZT22_16440) - 3401798..3403045 (+) 1248 WP_000414651.1 site-specific integrase -
  HZT22_RS16525 (HZT22_16445) - 3403038..3404570 (+) 1533 WP_001061276.1 site-specific integrase -
  HZT22_RS16530 (HZT22_16450) - 3404587..3406620 (+) 2034 WP_000807722.1 hypothetical protein -
  HZT22_RS16535 (HZT22_16455) - 3406613..3407032 (+) 420 WP_001062122.1 hypothetical protein -
  HZT22_RS16540 (HZT22_16460) avs4 3407108..3411871 (+) 4764 WP_000240574.1 AVAST type 4 anti-phage nuclease Avs4 -
  HZT22_RS16545 - 3412716..3413231 (-) 516 WP_240763255.1 abortive infection system antitoxin AbiGi family protein -
  HZT22_RS16550 (HZT22_16470) - 3413639..3413920 (-) 282 WP_001295689.1 YjcB family protein -
  HZT22_RS16555 (HZT22_16475) pdeC 3414350..3415936 (+) 1587 WP_000019548.1 c-di-GMP phosphodiesterase PdeC -
  HZT22_RS16560 (HZT22_16480) soxS 3415939..3416262 (-) 324 WP_000019358.1 superoxide response transcriptional regulator SoxS -
  HZT22_RS16565 (HZT22_16485) soxR 3416348..3416812 (+) 465 WP_000412428.1 redox-sensitive transcriptional activator SoxR -
  HZT22_RS16570 (HZT22_16490) ghxP 3417358..3418707 (+) 1350 WP_000106882.1 guanine/hypoxanthine transporter GhxP -
  HZT22_RS16575 (HZT22_16495) - 3418858..3420507 (+) 1650 WP_000402207.1 Na+/H+ antiporter -
  HZT22_RS16580 (HZT22_16500) espX5 3420661..3421953 (-) 1293 WP_001270095.1 T3SS effector pentapeptide repeat protein EspX5 -

Sequence


Protein


Download         Length: 178 a.a.        Molecular weight: 18975.00 Da        Isoelectric Point: 5.2358

>NTDB_id=465592 HZT22_RS16515 WP_000168305.1 3401103..3401639(+) (ssb) [Shigella flexneri strain STLEFF_27]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI
EGQLRTRKWTDQSGQDRYTTEVVVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
PAAPSNEPPMDFDDDIPF

Nucleotide


Download         Length: 537 bp        

>NTDB_id=465592 HZT22_RS16515 WP_000168305.1 3401103..3401639(+) (ssb) [Shigella flexneri strain STLEFF_27]
ATGGCCAGCAGAGGCGTAAACAAGGTTATTCTCGTTGGTAATCTGGGTCAGGACCCGGAAGTACGCTACATGCCAAATGG
TGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTG
AATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATC
GAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACACCACAGAAGTCGTGGTGAACGTTGG
CGGCACCATGCAGATGCTGGGTGGTCGTCAGGGTGGTGGCGCTCCGGCAGGTGGCAATATCGGTGGTGGTCAGCCGCAGG
GCGGTTGGGGTCAGCCTCAGCAGCCGCAGGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCCGCT
CCGGCAGCGCCGTCTAACGAGCCGCCGATGGACTTTGATGATGACATTCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

74.444

100

0.753

  ssb Glaesserella parasuis strain SC1401

57.923

100

0.596

  ssb Neisseria meningitidis MC58

48.066

100

0.489

  ssb Neisseria gonorrhoeae MS11

48.066

100

0.489