Detailed information NTG0465482

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JQ463_RS01950
• Genome accession: NZ_CP069330
• Coordinates: 419092..420240 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain R07
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 414092..425240

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQ463_RS01925 (JQ463_01925)	-	415192..415539 (-)	348	WP_124117681.1	DUF167 domain-containing protein	-
JQ463_RS01930 (JQ463_01930)	-	415536..416129 (-)	594	WP_003084544.1	YggT family protein	-
JQ463_RS01935 (JQ463_01935)	proC	416140..416961 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
JQ463_RS01940 (JQ463_01940)	-	416973..417665 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
JQ463_RS01945 (JQ463_01945)	pilT	417880..418914 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JQ463_RS01950 (JQ463_01950)	pilU	419092..420240 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
JQ463_RS01955 (JQ463_01955)	czcD	420248..421147 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JQ463_RS01960 (JQ463_01960)	-	421301..421705 (+)	405	WP_003084560.1	NINE protein	-
JQ463_RS01965 (JQ463_01965)	-	421948..423321 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
JQ463_RS01970 (JQ463_01970)	-	423318..424502 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=465482 JQ463_RS01950 WP_003100959.1 419092..420240(+) (pilU) [Pseudomonas aeruginosa strain R07]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=465482 JQ463_RS01950 WP_003100959.1 419092..420240(+) (pilU) [Pseudomonas aeruginosa strain R07]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382