Detailed information NTG0465072

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HYO64_RS14195
• Genome accession: NZ_CP058729
• Coordinates: 2961475..2962593 (+)
• Length: 372 a.a.
• NCBI ID: WP_114153513.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain M164-3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2956475..2967593

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HYO64_RS14185	-	2959591..2960283 (-)	693	WP_049068316.1	YggS family pyridoxal phosphate-dependent enzyme	-
HYO64_RS14190	pilT	2960410..2961447 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HYO64_RS14195	pilU	2961475..2962593 (+)	1119	WP_114153513.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HYO64_RS14200	fur	2962663..2963100 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HYO64_RS14205	-	2963212..2963610 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HYO64_RS14210	-	2963665..2963988 (-)	324	WP_114153511.1	RnfH family protein	-
HYO64_RS14215	-	2963988..2965055 (-)	1068	WP_004748097.1	hypothetical protein	-
HYO64_RS14220	-	2965208..2965663 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HYO64_RS14225	-	2965916..2966764 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41935.14 Da
• Isoelectric Point: 6.6944

>NTDB_id=465072 HYO64_RS14195 WP_114153513.1 2961475..2962593(+) (pilU) [Acinetobacter baumannii strain M164-3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADESPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=465072 HYO64_RS14195 WP_114153513.1 2961475..2962593(+) (pilU) [Acinetobacter baumannii strain M164-3]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAAGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.701	94.355	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392