Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   HYI45_RS00165 Genome accession   NZ_CP058714
Coordinates   16235..16708 (+) Length   157 a.a.
NCBI ID   WP_000168266.1    Uniprot ID   -
Organism   Escherichia coli strain M172-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 205..21597 16235..16708 within 0


Gene organization within MGE regions


Location: 205..21597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYI45_RS00010 - 205..1620 (-) 1416 WP_069912929.1 PBSX family phage terminase large subunit -
  HYI45_RS00015 - 1617..2057 (-) 441 WP_053287852.1 hypothetical protein -
  HYI45_RS00020 - 2060..2302 (-) 243 WP_000807788.1 DUF2560 family protein -
  HYI45_RS00025 - 2599..3126 (-) 528 WP_239794995.1 DUF2829 domain-containing protein -
  HYI45_RS00030 - 3208..3360 (-) 153 WP_039052544.1 hypothetical protein -
  HYI45_RS00035 - 3348..3785 (-) 438 WP_239794996.1 lysis protein -
  HYI45_RS00040 rrrD 3782..4279 (-) 498 WP_061353377.1 lysozyme RrrD -
  HYI45_RS00045 - 4257..4460 (-) 204 WP_000286100.1 phage holin family protein -
  HYI45_RS00050 - 4896..5519 (-) 624 WP_239794997.1 antitermination protein -
  HYI45_RS00055 - 5516..5704 (-) 189 WP_000994516.1 protein ninH -
  HYI45_RS00060 - 5701..6062 (-) 362 Protein_11 RusA family crossover junction endodeoxyribonuclease -
  HYI45_RS00065 - 6059..6349 (-) 291 WP_000002243.1 DUF1364 domain-containing protein -
  HYI45_RS00070 - 6349..7071 (-) 723 WP_001003986.1 DNA-binding protein -
  HYI45_RS00075 - 7064..7240 (-) 177 WP_000950962.1 protein NinF -
  HYI45_RS00080 - 7240..7599 (-) 360 WP_239794998.1 phage protein NinX family protein -
  HYI45_RS00085 - 7602..7778 (-) 177 WP_000113772.1 NinE family protein -
  HYI45_RS00090 ninD 7745..7918 (-) 174 WP_000984218.1 protein NinD -
  HYI45_RS00095 - 7915..8787 (-) 873 WP_000611491.1 phosphoadenosine phosphosulfate reductase family protein -
  HYI45_RS00100 - 8784..9224 (-) 441 WP_000736913.1 recombination protein NinB -
  HYI45_RS00105 - 9301..10737 (-) 1437 WP_001549089.1 DnaB-like helicase C-terminal domain-containing protein -
  HYI45_RS00110 - 10727..11626 (-) 900 WP_239794999.1 DNA replication protein -
  HYI45_RS00115 - 11619..11765 (-) 147 WP_000166207.1 DUF2740 family protein -
  HYI45_RS00120 - 11800..12078 (-) 279 WP_015966855.1 lambda phage CII family protein -
  HYI45_RS00125 - 12187..12372 (-) 186 WP_000276886.1 Cro/CI family transcriptional regulator -
  HYI45_RS00130 - 12453..13103 (+) 651 WP_000856967.1 LexA family transcriptional regulator -
  HYI45_RS00135 - 13960..14232 (+) 273 WP_012817806.1 hypothetical protein -
  HYI45_RS00140 - 14236..14460 (+) 225 WP_001077327.1 hypothetical protein -
  HYI45_RS00145 - 14523..14774 (+) 252 WP_000394305.1 hypothetical protein -
  HYI45_RS00150 - 15001..15135 (+) 135 WP_000972063.1 hypothetical protein -
  HYI45_RS00155 kil 15120..15272 (+) 153 WP_001459571.1 host cell division inhibitory peptide Kil -
  HYI45_RS00160 - 15527..16234 (+) 708 WP_239795000.1 Rad52/Rad22 family DNA repair protein -
  HYI45_RS00165 ssb 16235..16708 (+) 474 WP_000168266.1 single-stranded DNA-binding protein Machinery gene
  HYI45_RS00170 - 17211..17507 (+) 297 WP_049038791.1 phage anti-RecBCD protein -
  HYI45_RS00175 - 17518..17682 (+) 165 WP_001214456.1 DUF2737 family protein -
  HYI45_RS00180 - 17679..18128 (+) 450 WP_049038793.1 hypothetical protein -
  HYI45_RS00185 - 18125..18577 (+) 453 WP_049038794.1 ead/Ea22-like family protein -
  HYI45_RS26900 - 18531..18842 (+) 312 Protein_37 hypothetical protein -
  HYI45_RS26905 - 18831..19166 (+) 336 Protein_38 hypothetical protein -
  HYI45_RS00195 - 19163..19726 (+) 564 WP_239795001.1 DUF551 domain-containing protein -
  HYI45_RS00200 - 19823..20002 (+) 180 WP_001277767.1 Eag protein -
  HYI45_RS00205 - 20105..20449 (+) 345 WP_239795002.1 hypothetical protein -
  HYI45_RS00210 - 20529..20720 (+) 192 WP_000132739.1 AlpA family phage regulatory protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17608.44 Da        Isoelectric Point: 7.3192

>NTDB_id=464985 HYI45_RS00165 WP_000168266.1 16235..16708(+) (ssb) [Escherichia coli strain M172-1]
MASRGVNKVIIIGRLGHDPEIRYSPSGTAFANLTVATSEQWRDKQTGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYL
EGKLRTRKWQDQSGQDRFTTEVIVGVGGTMQMLGGKQGGNEQSSPQRNNGQQQRQQSQQQGNHSEPPMNFDDSDIPF

Nucleotide


Download         Length: 474 bp        

>NTDB_id=464985 HYI45_RS00165 WP_000168266.1 16235..16708(+) (ssb) [Escherichia coli strain M172-1]
ATGGCAAGCAGAGGCGTAAATAAGGTGATCATTATTGGTCGCCTTGGGCATGATCCAGAAATCAGATATTCACCATCAGG
AACGGCATTTGCAAACCTTACAGTTGCTACGTCAGAACAATGGCGTGATAAGCAAACTGGAGAGCAAAAGGAGCAGACGG
AGTGGCACCGTGTGGTAATGAGCGGGAAACTGGCAGAAATTGCCAGCGAATATCTGCGAAAAGGCTCTGAGGTTTATCTT
GAAGGCAAATTGCGGACAAGAAAATGGCAGGATCAAAGCGGACAGGATAGGTTCACTACCGAAGTCATCGTGGGCGTTGG
TGGAACCATGCAAATGCTTGGTGGCAAGCAAGGAGGCAATGAACAGTCTTCACCTCAGCGAAATAACGGTCAGCAACAAA
GACAGCAATCTCAGCAGCAGGGGAATCACAGCGAACCACCTATGAACTTCGACGATTCGGATATTCCGTTCTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(6-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

60.674

100

0.688
  ssb Glaesserella parasuis strain SC1401

50.549

100

0.586
  ssb Neisseria meningitidis MC58

41.243

100

0.465
  ssb Neisseria gonorrhoeae MS11

41.243

100

0.465