Detailed information NTG0464977

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JQ458_RS01945
• Genome accession: NZ_CP069325
• Coordinates: 418428..419576 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain R02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 413428..424576

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQ458_RS01920 (JQ458_01920)	-	414528..414875 (-)	348	WP_124117681.1	DUF167 domain-containing protein	-
JQ458_RS01925 (JQ458_01925)	-	414872..415465 (-)	594	WP_003084544.1	YggT family protein	-
JQ458_RS01930 (JQ458_01930)	proC	415476..416297 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
JQ458_RS01935 (JQ458_01935)	-	416309..417001 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
JQ458_RS01940 (JQ458_01940)	pilT	417216..418250 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JQ458_RS01945 (JQ458_01945)	pilU	418428..419576 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
JQ458_RS01950 (JQ458_01950)	czcD	419584..420483 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JQ458_RS01955 (JQ458_01955)	-	420637..421041 (+)	405	WP_003084560.1	NINE protein	-
JQ458_RS01960 (JQ458_01960)	-	421284..422657 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
JQ458_RS01965 (JQ458_01965)	-	422654..423838 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=464977 JQ458_RS01945 WP_003100959.1 418428..419576(+) (pilU) [Pseudomonas aeruginosa strain R02]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=464977 JQ458_RS01945 WP_003100959.1 418428..419576(+) (pilU) [Pseudomonas aeruginosa strain R02]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382