Detailed information NTG0464412

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JQM61_RS23070
• Genome accession: NZ_CP069198
• Coordinates: 4901896..4903044 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 152962
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4896896..4908044

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JQM61_RS23050 (JQM61_23050)	-	4897633..4898817 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
JQM61_RS23055 (JQM61_23055)	-	4898814..4900187 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
JQM61_RS23060 (JQM61_23060)	-	4900430..4900834 (-)	405	WP_003084560.1	NINE protein	-
JQM61_RS23065 (JQM61_23065)	czcD	4900989..4901888 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
JQM61_RS23070 (JQM61_23070)	pilU	4901896..4903044 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
JQM61_RS23075 (JQM61_23075)	pilT	4903222..4904256 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JQM61_RS23080 (JQM61_23080)	-	4904471..4905163 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
JQM61_RS23085 (JQM61_23085)	proC	4905175..4905996 (+)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
JQM61_RS23090 (JQM61_23090)	-	4906007..4906600 (+)	594	WP_003084544.1	YggT family protein	-
JQM61_RS23095 (JQM61_23095)	-	4906597..4906893 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=464412 JQM61_RS23070 WP_003100959.1 4901896..4903044(-) (pilU) [Pseudomonas aeruginosa strain 152962]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=464412 JQM61_RS23070 WP_003100959.1 4901896..4903044(-) (pilU) [Pseudomonas aeruginosa strain 152962]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382