Detailed information NTG0464256

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IS492_RS02015
• Genome accession: NZ_CP069177
• Coordinates: 434505..435653 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Z154
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 429505..440653

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IS492_RS01990 (IS492_01995)	-	430656..430952 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IS492_RS01995 (IS492_02000)	-	430949..431542 (-)	594	WP_003084544.1	YggT family protein	-
IS492_RS02000 (IS492_02005)	proC	431553..432374 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
IS492_RS02005 (IS492_02010)	-	432386..433078 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IS492_RS02010 (IS492_02015)	pilT	433293..434327 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IS492_RS02015 (IS492_02020)	pilU	434505..435653 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
IS492_RS02020 (IS492_02025)	czcD	435661..436560 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IS492_RS02025 (IS492_02030)	-	436714..437118 (+)	405	WP_003084560.1	NINE protein	-
IS492_RS02030 (IS492_02035)	-	437361..438734 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
IS492_RS02035 (IS492_02040)	-	438731..439915 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=464256 IS492_RS02015 WP_003100959.1 434505..435653(+) (pilU) [Pseudomonas aeruginosa strain Z154]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=464256 IS492_RS02015 WP_003100959.1 434505..435653(+) (pilU) [Pseudomonas aeruginosa strain Z154]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382