Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HWQ57_RS29090 Genome accession   NZ_CP058333
Coordinates   6234495..6235559 (+) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ACR20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6229495..6240559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HWQ57_RS29080 (HWQ57_28880) - 6230466..6231734 (+) 1269 WP_003103278.1 malic enzyme -
  HWQ57_RS29085 (HWQ57_28885) - 6231839..6234310 (-) 2472 WP_179118826.1 penicillin-binding protein 1A -
  HWQ57_RS29090 (HWQ57_28890) pilM 6234495..6235559 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HWQ57_RS29095 (HWQ57_28895) pilN 6235559..6236155 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  HWQ57_RS29100 (HWQ57_28900) pilO 6236152..6236775 (+) 624 WP_023110484.1 type 4a pilus biogenesis protein PilO -
  HWQ57_RS29105 (HWQ57_28905) pilP 6236772..6237296 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  HWQ57_RS29110 (HWQ57_28910) pilQ 6237350..6239482 (+) 2133 WP_023110483.1 type 4a pilus secretin PilQ Machinery gene
  HWQ57_RS29115 (HWQ57_28915) aroK 6239494..6240012 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=463903 HWQ57_RS29090 WP_003110888.1 6234495..6235559(+) (pilM) [Pseudomonas aeruginosa strain ACR20]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=463903 HWQ57_RS29090 WP_003110888.1 6234495..6235559(+) (pilM) [Pseudomonas aeruginosa strain ACR20]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466