Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EF62_RS02270 Genome accession   NC_017312
Coordinates   469345..470715 (+) Length   456 a.a.
NCBI ID   WP_002356050.1    Uniprot ID   -
Organism   Enterococcus faecalis 62     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 464345..475715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EF62_RS02245 (EF62_0425) - 464835..465302 (-) 468 WP_002381334.1 VOC family protein -
  EF62_RS02250 (EF62_0426) - 465367..466314 (+) 948 WP_002385468.1 YafY family protein -
  EF62_RS02255 (EF62_0427) - 466406..467659 (-) 1254 WP_025189719.1 glutamate-5-semialdehyde dehydrogenase -
  EF62_RS02260 (EF62_0428) proB 467646..468461 (-) 816 WP_002389550.1 glutamate 5-kinase -
  EF62_RS02265 (EF62_0429) - 468781..469266 (+) 486 WP_002356049.1 dUTP diphosphatase -
  EF62_RS02270 (EF62_0430) radA 469345..470715 (+) 1371 WP_002356050.1 DNA repair protein RadA Machinery gene
  EF62_RS02275 (EF62_0431) - 470816..471961 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  EF62_RS02280 (EF62_0432) ispF 471980..472453 (+) 474 WP_002356052.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EF62_RS02285 (EF62_0433) gltX 472506..473963 (+) 1458 WP_002381335.1 glutamate--tRNA ligase -
  EF62_RS02295 (EF62_0434) epsC 474233..474772 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49757.28 Da        Isoelectric Point: 7.3489

>NTDB_id=46083 EF62_RS02270 WP_002356050.1 469345..470715(+) (radA) [Enterococcus faecalis 62]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=46083 EF62_RS02270 WP_002356050.1 469345..470715(+) (radA) [Enterococcus faecalis 62]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTTTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATTTCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCGATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTATTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCAGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656

Multiple sequence alignment