Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   JKV47_RS09020 Genome accession   NZ_CP068680
Coordinates   1874926..1876419 (+) Length   497 a.a.
NCBI ID   WP_000249377.1    Uniprot ID   -
Organism   Escherichia coli strain NCCP12480     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1869926..1881419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKV47_RS08995 (JKV47_08915) gss 1869976..1871835 (+) 1860 WP_001354280.1 bifunctional glutathionylspermidine amidase/synthase -
  JKV47_RS09000 (JKV47_08920) - 1872127..1872357 (+) 231 Protein_1750 inorganic phosphate transporter -
  JKV47_RS09005 (JKV47_08925) - 1872413..1873110 (+) 698 WP_223419586.1 IS1 family transposase -
  JKV47_RS09010 (JKV47_08930) gspC 1873186..1874166 (+) 981 WP_115194747.1 type II secretion system protein GspC -
  JKV47_RS09015 (JKV47_08935) - 1874141..1874926 (+) 786 Protein_1753 type II secretion system protein GspD -
  JKV47_RS09020 (JKV47_08940) pilB/pilB1 1874926..1876419 (+) 1494 WP_000249377.1 type II secretion system ATPase GspE Machinery gene
  JKV47_RS09025 (JKV47_08945) gspF 1876419..1877642 (+) 1224 WP_001535576.1 type II secretion system inner membrane protein GspF -
  JKV47_RS09030 (JKV47_08950) gspG 1877659..1878114 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  JKV47_RS09035 (JKV47_08955) gspH 1878118..1878681 (+) 564 WP_001115115.1 type II secretion system minor pseudopilin GspH -
  JKV47_RS09040 (JKV47_08960) gspI 1878678..1879049 (+) 372 WP_000820118.1 type II secretion system minor pseudopilin GspI -
  JKV47_RS09045 (JKV47_08965) gspJ 1879046..1879651 (+) 606 WP_001354285.1 type II secretion system minor pseudopilin GspJ -
  JKV47_RS09050 (JKV47_08970) - 1879648..1880295 (+) 648 WP_235144911.1 type II secretion system protein GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55194.32 Da        Isoelectric Point: 6.0390

>NTDB_id=460623 JKV47_RS09020 WP_000249377.1 1874926..1876419(+) (pilB/pilB1) [Escherichia coli strain NCCP12480]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=460623 JKV47_RS09020 WP_000249377.1 1874926..1876419(+) (pilB/pilB1) [Escherichia coli strain NCCP12480]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATTCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGACTGCCGCCAGCCCGCACCAGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.615

79.879

0.388
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362