Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HV135_RS01705 Genome accession   NZ_CP056647
Coordinates   352107..352631 (+) Length   174 a.a.
NCBI ID   WP_003826621.1    Uniprot ID   A0A5B0T4S2
Organism   Citrobacter sp. RHBSTW-00229     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 298304..360721 352107..352631 within 0


Gene organization within MGE regions


Location: 298304..360721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV135_RS01375 (HV135_01370) - 298304..298576 (-) 273 WP_020838472.1 pyocin activator PrtN family protein -
  HV135_RS01380 (HV135_01375) - 298615..299187 (-) 573 WP_181632366.1 3'-5' exonuclease -
  HV135_RS01385 (HV135_01380) - 299209..299463 (-) 255 WP_053390038.1 DUF4222 domain-containing protein -
  HV135_RS25930 - 299487..299756 (-) 270 Protein_266 DUF550 domain-containing protein -
  HV135_RS01395 (HV135_01390) - 299927..300349 (-) 423 WP_181632367.1 HNH endonuclease -
  HV135_RS01400 (HV135_01395) - 300351..300863 (-) 513 Protein_268 hypothetical protein -
  HV135_RS01405 (HV135_01400) - 300887..301141 (-) 255 WP_181632369.1 hypothetical protein -
  HV135_RS01410 (HV135_01405) - 301138..301314 (-) 177 WP_001253787.1 hypothetical protein -
  HV135_RS01415 (HV135_01410) - 301302..301841 (-) 540 WP_097475729.1 hypothetical protein -
  HV135_RS01420 (HV135_01415) rdgC 301949..302866 (-) 918 WP_181632370.1 recombination-associated protein RdgC -
  HV135_RS01425 (HV135_01420) - 303505..303738 (+) 234 WP_001058060.1 hypothetical protein -
  HV135_RS01430 (HV135_01425) - 304061..304693 (-) 633 WP_000381890.1 S24 family peptidase -
  HV135_RS01435 (HV135_01430) - 304794..304997 (+) 204 WP_000548758.1 Cro/CI family transcriptional regulator -
  HV135_RS01440 (HV135_01435) - 305021..305572 (+) 552 WP_000526199.1 YmfL family putative regulatory protein -
  HV135_RS01445 (HV135_01440) - 305745..305930 (+) 186 WP_181632371.1 DUF4222 domain-containing protein -
  HV135_RS25935 - 305914..306936 (+) 1023 WP_249418135.1 hypothetical protein -
  HV135_RS01455 (HV135_01450) - 306936..307832 (+) 897 WP_181632464.1 phage N-6-adenine-methyltransferase -
  HV135_RS01460 (HV135_01455) - 307825..309759 (+) 1935 WP_181632372.1 DNA cytosine methyltransferase -
  HV135_RS01465 (HV135_01460) - 309756..310154 (+) 399 WP_000780154.1 RusA family crossover junction endodeoxyribonuclease -
  HV135_RS01470 (HV135_01465) - 310241..311128 (+) 888 WP_249418136.1 ORF6N domain-containing protein -
  HV135_RS01475 (HV135_01470) - 311125..312120 (+) 996 WP_181632373.1 DUF968 domain-containing protein -
  HV135_RS01480 (HV135_01475) - 312142..312831 (+) 690 WP_181632374.1 bacteriophage antitermination protein Q -
  HV135_RS01485 (HV135_01480) - 313041..313322 (+) 282 WP_181632375.1 hypothetical protein -
  HV135_RS01490 (HV135_01485) - 313410..313796 (+) 387 WP_061353905.1 phage holin family protein -
  HV135_RS01495 (HV135_01490) - 313783..314064 (+) 282 WP_181632376.1 phage holin family protein -
  HV135_RS01500 (HV135_01495) - 314220..314834 (+) 615 WP_181632377.1 glycoside hydrolase family 19 protein -
  HV135_RS01505 (HV135_01500) - 314842..315111 (+) 270 WP_181632378.1 hypothetical protein -
  HV135_RS25940 - 315068..315265 (+) 198 WP_249418157.1 hypothetical protein -
  HV135_RS01515 (HV135_01510) - 315463..316026 (+) 564 WP_181632466.1 phage antirepressor N-terminal domain-containing protein -
  HV135_RS01520 (HV135_01515) - 316116..316313 (+) 198 WP_000219369.1 hypothetical protein -
  HV135_RS01525 (HV135_01520) - 316695..317423 (+) 729 WP_181632379.1 Fur-regulated protein -
  HV135_RS01530 (HV135_01525) - 317674..318219 (+) 546 WP_057065046.1 terminase small subunit -
  HV135_RS01535 (HV135_01530) - 318194..320116 (+) 1923 WP_057065045.1 phage terminase large subunit family protein -
  HV135_RS01540 (HV135_01535) - 320116..320322 (+) 207 WP_003034782.1 gpW family protein -
  HV135_RS01545 (HV135_01540) - 320319..321911 (+) 1593 WP_003826190.1 phage portal protein -
  HV135_RS01550 (HV135_01545) - 321892..323229 (+) 1338 WP_136397966.1 S49 family peptidase -
  HV135_RS01555 (HV135_01550) - 323239..323571 (+) 333 WP_181632380.1 head decoration protein -
  HV135_RS01560 (HV135_01555) - 323639..324664 (+) 1026 WP_003034798.1 major capsid protein -
  HV135_RS01565 (HV135_01560) gpFI 324710..325114 (+) 405 WP_057065033.1 DNA-packaging protein FI -
  HV135_RS01570 (HV135_01565) - 325126..325479 (+) 354 WP_057065034.1 head-tail joining protein -
  HV135_RS01575 (HV135_01570) - 325489..326043 (+) 555 WP_057065035.1 phage tail protein -
  HV135_RS01580 (HV135_01575) - 326040..326438 (+) 399 WP_057065036.1 phage minor tail U family protein -
  HV135_RS01585 (HV135_01580) - 326446..327183 (+) 738 WP_181632381.1 phage tail protein -
  HV135_RS01590 (HV135_01585) - 327221..327628 (+) 408 WP_181632382.1 phage minor tail protein G -
  HV135_RS01595 (HV135_01590) - 327679..327957 (+) 279 WP_008322441.1 phage tail assembly protein T -
  HV135_RS01600 (HV135_01595) - 327935..330454 (+) 2520 WP_181632383.1 phage tail tape measure protein -
  HV135_RS01605 (HV135_01600) - 330458..330805 (+) 348 WP_125363803.1 phage tail protein -
  HV135_RS01610 (HV135_01605) - 330802..331557 (+) 756 WP_181632384.1 phage minor tail protein L -
  HV135_RS01615 (HV135_01610) - 331559..332269 (+) 711 WP_049001720.1 C40 family peptidase -
  HV135_RS01620 (HV135_01615) - 332301..332723 (+) 423 WP_181632385.1 hypothetical protein -
  HV135_RS01625 (HV135_01620) - 332758..333348 (+) 591 WP_048217469.1 tail assembly protein -
  HV135_RS01630 (HV135_01625) - 333403..336606 (+) 3204 WP_181632386.1 host specificity protein J -
  HV135_RS01635 (HV135_01630) - 336608..337552 (+) 945 WP_181632387.1 DUF6453 family protein -
  HV135_RS01640 (HV135_01635) - 337562..338866 (+) 1305 WP_181632388.1 phage tail protein -
  HV135_RS01645 (HV135_01640) - 339008..339250 (-) 243 WP_000497432.1 DinI family protein -
  HV135_RS01650 (HV135_01645) - 339272..339718 (+) 447 WP_077909674.1 S24 family peptidase -
  HV135_RS01655 (HV135_01650) - 339751..340845 (-) 1095 WP_149911819.1 site-specific integrase -
  HV135_RS01660 (HV135_01655) dusA 340933..341970 (+) 1038 WP_181632389.1 tRNA dihydrouridine(20/20a) synthase DusA -
  HV135_RS01665 (HV135_01660) pspG 342104..342346 (+) 243 WP_016155352.1 envelope stress response protein PspG -
  HV135_RS01670 (HV135_01665) - 342579..343562 (-) 984 WP_141876101.1 quinone oxidoreductase -
  HV135_RS01675 (HV135_01670) dnaB 343627..345042 (+) 1416 WP_003826610.1 replicative DNA helicase -
  HV135_RS01680 (HV135_01675) alr 345090..346169 (+) 1080 WP_016155354.1 alanine racemase -
  HV135_RS01685 (HV135_01680) tyrB 346299..347492 (+) 1194 WP_181221087.1 aromatic amino acid transaminase -
  HV135_RS01690 (HV135_01685) aphA 347742..348455 (+) 714 WP_003826615.1 acid phosphatase AphA -
  HV135_RS01695 (HV135_01690) - 348582..348938 (+) 357 WP_016155357.1 MmcQ/YjbR family DNA-binding protein -
  HV135_RS01700 (HV135_01695) uvrA 349033..351855 (-) 2823 WP_016155358.1 excinuclease ABC subunit UvrA -
  HV135_RS01705 (HV135_01700) ssb 352107..352631 (+) 525 WP_003826621.1 single-stranded DNA-binding protein SSB1 Machinery gene
  HV135_RS01710 (HV135_01705) - 352698..352979 (-) 282 WP_016155360.1 YjcB family protein -
  HV135_RS01715 (HV135_01710) - 353531..355117 (+) 1587 WP_047499546.1 EAL domain-containing protein -
  HV135_RS01720 (HV135_01715) soxS 355120..355443 (-) 324 WP_016155362.1 superoxide response transcriptional regulator SoxS -
  HV135_RS01725 (HV135_01720) soxR 355530..355988 (+) 459 WP_016155363.1 redox-sensitive transcriptional activator SoxR -
  HV135_RS01730 (HV135_01725) ghxP 356593..357942 (+) 1350 WP_016155449.1 guanine/hypoxanthine transporter GhxP -
  HV135_RS01735 (HV135_01730) - 358100..359746 (+) 1647 WP_016155450.1 Na+/H+ antiporter -
  HV135_RS01740 (HV135_01735) - 359831..360721 (-) 891 WP_016155366.1 LysR family transcriptional regulator -

Sequence


Protein


Download         Length: 174 a.a.        Molecular weight: 18621.66 Da        Isoelectric Point: 5.2456

>NTDB_id=460071 HV135_RS01705 WP_003826621.1 352107..352631(+) (ssb) [Citrobacter sp. RHBSTW-00229]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI
EGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAP
SNEPPMDFDDDIPF

Nucleotide


Download         Length: 525 bp        

>NTDB_id=460071 HV135_RS01705 WP_003826621.1 352107..352631(+) (ssb) [Citrobacter sp. RHBSTW-00229]
ATGGCCAGCAGAGGCGTAAACAAGGTGATTCTCGTCGGTAATCTGGGCCAGGACCCGGAAGTACGCTATATGCCGAATGG
TGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAAATGAAAGAGCAGACTG
AATGGCACCGTGTGGTGCTGTTCGGCAAACTGGCGGAAGTTGCCAGCGAATATCTGCGTAAAGGTTCCCAGGTCTATATC
GAAGGTCAGCTGCGTACCCGCAAATGGACCGATCAATCCGGCGTAGAAAAGTACACCACTGAAGTTGTGGTTAACGTTGG
CGGCACCATGCAAATGCTGGGCGGTCGTCAGGGTGGTGGTGCTCCGGCAGGTGGCGGCCAGCAGCAGGGTGGTTGGGGTC
AGCCTCAGCAGCCGCAAGGTGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGCCCGCAGCAGTCGGCTCCGGCAGCACCG
TCTAATGAGCCGCCGATGGATTTCGACGACGATATTCCGTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A5B0T4S2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

73.889

100

0.764

  ssb Glaesserella parasuis strain SC1401

56.831

100

0.598

  ssb Neisseria meningitidis MC58

48.603

100

0.5

  ssb Neisseria gonorrhoeae MS11

48.603

100

0.5