Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JHW37_RS26945 Genome accession   NZ_CP068238
Coordinates   5765624..5766247 (-) Length   207 a.a.
NCBI ID   WP_003148775.1    Uniprot ID   A0A2R3IMZ4
Organism   Pseudomonas aeruginosa strain A39-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5760624..5771247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW37_RS26925 (JHW37_26925) aroB 5761219..5762325 (-) 1107 WP_012077719.1 3-dehydroquinate synthase -
  JHW37_RS26930 (JHW37_26930) aroK 5762375..5762893 (-) 519 WP_003148769.1 shikimate kinase AroK -
  JHW37_RS26935 (JHW37_26935) pilQ 5762905..5765049 (-) 2145 WP_003148771.1 type 4a pilus secretin PilQ Machinery gene
  JHW37_RS26940 (JHW37_26940) comL/pilP 5765103..5765627 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  JHW37_RS26945 (JHW37_26945) pilO 5765624..5766247 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHW37_RS26950 (JHW37_26950) pilN 5766244..5766840 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN Machinery gene
  JHW37_RS26955 (JHW37_26955) pilM 5766840..5767904 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  JHW37_RS26960 (JHW37_26960) - 5768089..5770560 (+) 2472 WP_012077723.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22907.32 Da        Isoelectric Point: 4.6862

>NTDB_id=459269 JHW37_RS26945 WP_003148775.1 5765624..5766247(-) (pilO) [Pseudomonas aeruginosa strain A39-1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVEPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=459269 JHW37_RS26945 WP_003148775.1 5765624..5766247(-) (pilO) [Pseudomonas aeruginosa strain A39-1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTGCTTGCGCTGGGCTACAACTTCCACCTGAGCGACA
TGCAGGCGCAGCTGGAGCAGCAGGCCGCGGAGGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCGCAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAACTGCCCAGCGACAC
CGAGGTGCCCGGCCTGCTCGAGGACATCACTCGCACCGGACTGGGCAGCGGGCTGGAGTTCGAGGAGATCAAGCTGCTCC
CCGAGGTCACCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGTGGCTATCACGACCTGGCGACCTTT
GTCAGCGGCGTCTCCAGTCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGAGCCCGGCAGTACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IMZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

42.857

91.304

0.391