Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   JHW37_RS26950 Genome accession   NZ_CP068238
Coordinates   5766244..5766840 (-) Length   198 a.a.
NCBI ID   WP_003148777.1    Uniprot ID   A0A2R3J064
Organism   Pseudomonas aeruginosa strain A39-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5761244..5771840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW37_RS26930 (JHW37_26930) aroK 5762375..5762893 (-) 519 WP_003148769.1 shikimate kinase AroK -
  JHW37_RS26935 (JHW37_26935) pilQ 5762905..5765049 (-) 2145 WP_003148771.1 type 4a pilus secretin PilQ Machinery gene
  JHW37_RS26940 (JHW37_26940) comL/pilP 5765103..5765627 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  JHW37_RS26945 (JHW37_26945) pilO 5765624..5766247 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO Machinery gene
  JHW37_RS26950 (JHW37_26950) pilN 5766244..5766840 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN Machinery gene
  JHW37_RS26955 (JHW37_26955) pilM 5766840..5767904 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  JHW37_RS26960 (JHW37_26960) - 5768089..5770560 (+) 2472 WP_012077723.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22217.54 Da        Isoelectric Point: 10.3054

>NTDB_id=459270 JHW37_RS26950 WP_003148777.1 5766244..5766840(-) (pilN) [Pseudomonas aeruginosa strain A39-1]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGRNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEQDAKAKNGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=459270 JHW37_RS26950 WP_003148777.1 5766244..5766840(-) (pilN) [Pseudomonas aeruginosa strain A39-1]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATCCTCGGTGG
CGTGCTGGTGGCCTCGGCGGCCCTGGTGTTCCTCGGCGACCAGTACTTCACCGCCGCCATCGAGAACCAGAATGCGCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTGCTGGACGCGCGGATCAAGGAGATCAGCGAGCTGAAGTCGCGCCGCCAGCAG
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGCCCGATCATCGGCCGGGTGTTCGACCAGCTCGTGCG
CACGCTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAGGAATATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGTGTCTCCAACCTGATGCGCAACATGGACGCGTCCGAATGGCTGACCGCGCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGGGCGGTGGACCAGGCCAACGTGTTCCAGTTGACCGTGCAGCAGACGCAGCCGGGCGAGCAGGATGCCAA
GGCCAAGAATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3J064

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369