Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   HV561_RS05160 Genome accession   NZ_CP055302
Coordinates   1009350..1010663 (-) Length   437 a.a.
NCBI ID   WP_176808050.1    Uniprot ID   -
Organism   Mannheimia pernigra strain 16CN0041     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1004350..1015663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV561_RS05130 (HV561_05130) ruvB 1004979..1005983 (-) 1005 WP_176808046.1 Holliday junction branch migration DNA helicase RuvB -
  HV561_RS05135 (HV561_05135) ruvA 1005994..1006596 (-) 603 WP_176808047.1 Holliday junction branch migration protein RuvA -
  HV561_RS05140 (HV561_05140) - 1006705..1007331 (-) 627 WP_159629280.1 LysE family transporter -
  HV561_RS05145 (HV561_05145) - 1007325..1007807 (-) 483 WP_176808048.1 phosphatidylglycerophosphatase A -
  HV561_RS05150 (HV561_05150) thiL 1007813..1008793 (-) 981 WP_176808049.1 thiamine-phosphate kinase -
  HV561_RS05155 (HV561_05155) nusB 1008857..1009270 (-) 414 WP_159629283.1 transcription antitermination factor NusB -
  HV561_RS05160 (HV561_05160) comE 1009350..1010663 (-) 1314 WP_176808050.1 type IV pilus secretin PilQ Machinery gene
  HV561_RS05165 (HV561_05165) - 1010672..1011082 (-) 411 WP_176808051.1 hypothetical protein -
  HV561_RS05170 (HV561_05170) - 1011084..1011617 (-) 534 WP_176808052.1 hypothetical protein -
  HV561_RS05175 (HV561_05175) - 1011619..1012140 (-) 522 WP_176808053.1 hypothetical protein -
  HV561_RS05180 (HV561_05180) - 1012116..1012826 (-) 711 WP_176808054.1 competence protein ComA -
  HV561_RS05185 (HV561_05185) - 1012953..1015502 (+) 2550 WP_176808055.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 48453.69 Da        Isoelectric Point: 8.9264

>NTDB_id=457480 HV561_RS05160 WP_176808050.1 1009350..1010663(-) (comE) [Mannheimia pernigra strain 16CN0041]
MFRIFLFLFISSSLAFANTISLAVKNAPTATILTYLSEEIGKNIVLDDNIQTKSTLRLENKSIDDIFKVISTMNKLSLHQ
EGDVIYISKKEEKLTDLKTIPIAHLQNNIENTPLVTTPKLITKTIKLHYAKASEVIESLTKGSGTFLSENGYIYFDERSN
SLIIKDSTKSLQHIEKLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGMFSKGADHYKFGGRLESNGLSNVANNLNVS
FPVTNGASAVLQVASINSRVLDLELSALEQENSVEIIASPRLLTTNKKPASIKQGTEIPYVMYNKKSEATNVEFKEAVLG
LEVIPHLSTENQILLDLIVTQNSPNSQSGSSGLVTIDKQELNTQVFAKHGETIVLGGVFQHLTQKGEDKVPILGSIPFIK
RLFSQTRDKISKRELVIFVTPYIIQSNEKLGGVKKSK

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=457480 HV561_RS05160 WP_176808050.1 1009350..1010663(-) (comE) [Mannheimia pernigra strain 16CN0041]
ATGTTTAGAATTTTTCTTTTTTTATTTATTAGCTCCTCATTAGCATTTGCTAACACAATTTCTCTTGCTGTGAAAAATGC
CCCCACTGCAACCATTTTGACTTATTTATCCGAAGAAATAGGAAAAAATATTGTTTTAGATGATAATATTCAAACAAAAT
CAACATTAAGATTAGAAAATAAATCCATTGACGATATATTCAAAGTAATAAGCACAATGAATAAATTATCGCTACATCAA
GAAGGTGATGTTATTTATATCAGTAAAAAAGAAGAAAAATTGACTGATTTAAAAACAATTCCAATCGCTCATTTGCAAAA
TAATATAGAAAATACACCGCTTGTTACCACACCCAAACTCATTACTAAAACAATAAAACTACATTATGCTAAAGCCTCAG
AAGTGATTGAATCTTTAACAAAAGGCAGCGGTACTTTTTTATCTGAAAATGGCTATATCTATTTTGATGAGCGAAGTAAT
AGTTTAATTATCAAAGACAGTACAAAATCACTTCAACACATAGAAAAGTTAGTTAAACAGCTTGACCAACCAACTGAGCA
AATTGCAATTGAAGCACGCATTGTCACAATCAGCAGTGAACATTTACAAGAATTGGGGGTGCGTTGGGGAATGTTCTCAA
AAGGGGCTGATCACTATAAATTTGGTGGGCGACTTGAAAGTAACGGATTAAGCAATGTTGCTAATAATCTCAATGTGAGT
TTTCCTGTTACGAATGGTGCTTCAGCAGTTTTACAAGTCGCTTCTATTAATAGCCGAGTACTGGATCTGGAATTGAGTGC
CTTAGAGCAAGAAAATAGTGTAGAAATTATCGCCAGCCCTCGCCTTTTGACTACGAATAAAAAACCAGCGAGTATCAAAC
AAGGGACAGAAATTCCTTATGTGATGTACAATAAAAAATCGGAAGCTACTAATGTGGAATTTAAAGAAGCCGTGCTGGGC
TTAGAAGTTATTCCTCATCTTTCGACTGAAAATCAAATATTACTCGATCTGATTGTTACTCAAAATTCTCCTAATTCGCA
ATCAGGCAGTAGTGGCTTAGTGACTATTGATAAACAAGAATTGAATACACAAGTTTTTGCGAAACACGGCGAAACGATCG
TTTTAGGCGGAGTTTTCCAACATTTAACGCAAAAAGGAGAAGATAAAGTACCTATTTTAGGTTCAATTCCCTTTATCAAA
CGCTTATTTAGCCAAACTCGAGATAAAATTAGCAAAAGAGAACTTGTGATTTTTGTTACCCCTTACATTATTCAATCAAA
TGAAAAATTAGGTGGTGTGAAAAAATCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(267-425)

Bacterial type II/III secretion system short domain (Secretin_N)

(122-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

61.379

99.542

0.611
  comE Haemophilus influenzae Rd KW20

49.206

100

0.497
  comE Haemophilus influenzae 86-028NP

48.98

100

0.494
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.623

97.025

0.384
  pilQ Vibrio cholerae strain A1552

39.623

97.025

0.384
  pilQ Vibrio campbellii strain DS40M4

38.462

98.169

0.378