Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JJB18_RS04925 Genome accession   NZ_CP067241
Coordinates   1035282..1036262 (+) Length   326 a.a.
NCBI ID   WP_072693229.1    Uniprot ID   -
Organism   Escherichia coli strain P19_598     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030282..1041262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJB18_RS04895 (JJB18_04895) yggM 1030887..1031834 (+) 948 WP_072693227.1 DUF1202 family protein -
  JJB18_RS04900 (JJB18_04900) hemW 1031959..1033095 (-) 1137 WP_000239953.1 radical SAM family heme chaperone HemW -
  JJB18_RS04905 (JJB18_04905) rdgB 1033088..1033681 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  JJB18_RS04910 (JJB18_04910) yggU 1033689..1033979 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  JJB18_RS04915 (JJB18_04915) yggT 1033976..1034542 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JJB18_RS04920 (JJB18_04920) yggS 1034560..1035264 (-) 705 WP_000997804.1 pyridoxal phosphate homeostasis protein -
  JJB18_RS04925 (JJB18_04925) pilT 1035282..1036262 (+) 981 WP_072693229.1 type IV pilus twitching motility protein PilT Machinery gene
  JJB18_RS04930 (JJB18_04930) ruvX 1036436..1036852 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  JJB18_RS04935 (JJB18_04935) yqgE 1036852..1037415 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  JJB18_RS04940 (JJB18_04940) gshB 1037524..1038474 (-) 951 WP_000593273.1 glutathione synthase -
  JJB18_RS04945 (JJB18_04945) rsmE 1038487..1039218 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JJB18_RS04950 (JJB18_04950) endA 1039298..1040005 (-) 708 WP_023149797.1 deoxyribonuclease I -
  JJB18_RS04955 (JJB18_04955) yggI 1040100..1040597 (-) 498 Protein_987 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35975.11 Da        Isoelectric Point: 5.9975

>NTDB_id=457301 JJB18_RS04925 WP_072693229.1 1035282..1036262(+) (pilT) [Escherichia coli strain P19_598]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAGLSQKLEVDKQEGRVALFESLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=457301 JJB18_RS04925 WP_072693229.1 1035282..1036262(+) (pilT) [Escherichia coli strain P19_598]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGGGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATCACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilB Legionella pneumophila strain ERS1305867

31.481

100

0.365
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362