Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HUW35_RS05455 Genome accession   NZ_CP055157
Coordinates   1296316..1297392 (-) Length   358 a.a.
NCBI ID   WP_181254612.1    Uniprot ID   A0A7H8PWW3
Organism   Microbulbifer sp. YPW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1291316..1302392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW35_RS05430 (HUW35_05435) aroK 1291829..1292350 (-) 522 WP_219932692.1 shikimate kinase AroK -
  HUW35_RS05435 (HUW35_05440) pilQ 1292350..1294575 (-) 2226 WP_181254608.1 type IV pilus secretin PilQ family protein -
  HUW35_RS05440 (HUW35_05445) - 1294578..1295105 (-) 528 WP_181254609.1 pilus assembly protein PilP -
  HUW35_RS05445 (HUW35_05450) - 1295102..1295722 (-) 621 WP_181254610.1 type 4a pilus biogenesis protein PilO -
  HUW35_RS05450 (HUW35_05455) - 1295725..1296312 (-) 588 WP_181254611.1 PilN domain-containing protein -
  HUW35_RS05455 (HUW35_05460) pilM 1296316..1297392 (-) 1077 WP_181254612.1 pilus assembly protein PilM Machinery gene
  HUW35_RS05460 (HUW35_05465) - 1297549..1300236 (+) 2688 WP_181254613.1 penicillin-binding protein 1A -
  HUW35_RS05465 (HUW35_05470) - 1300314..1300799 (-) 486 WP_181254614.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38917.15 Da        Isoelectric Point: 4.3312

>NTDB_id=456436 HUW35_RS05455 WP_181254612.1 1296316..1297392(-) (pilM) [Microbulbifer sp. YPW1]
MGMLPFLNKGPKAMLGLDISSTSVKLLELSRSGDKYRVESYATEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLDMPADLSDDALEAQIALEADQYIPYPLDEVNLDFEVQGPSEKGEGQVEVLLAACRSENVEQRISAL
GEADLQPGVVDVEAYAIERAYTLLDNQFPPQEQLVVAVIDIGATMSALSVMVDGRTVYTREQLFGGRQLTDEIQRRYGLS
AEEATLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMDGLAELVGEKLNKPTMV
ANPFRDMSVASRVNRQALASEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=456436 HUW35_RS05455 WP_181254612.1 1296316..1297392(-) (pilM) [Microbulbifer sp. YPW1]
ATGGGGATGCTCCCTTTTCTCAATAAGGGCCCGAAAGCAATGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
CGAACTGAGTCGCAGTGGCGACAAATACCGCGTGGAAAGCTATGCAACCGAGCCCCTGCCTCCGAATGCGGTGGTGGATA
AAAATATCAATGATGTGGGAGCCATCGCCGAGGCGATTCGCAAGGTGGTGCGCCGCTCCAAGACCCGGCTGCGGCAGGCG
GCCGTGGCGGTTTCCGGCTCTGCGGTCATTACCAAGACCCTGGATATGCCCGCCGATCTCTCCGACGATGCGCTGGAAGC
CCAGATCGCACTGGAAGCCGACCAGTACATTCCCTACCCCCTGGACGAGGTAAACCTCGACTTTGAAGTGCAGGGGCCGT
CCGAAAAGGGTGAGGGGCAGGTTGAAGTGCTGCTGGCGGCATGCCGCAGCGAGAATGTAGAGCAGCGGATTTCCGCGCTG
GGCGAAGCCGATCTCCAGCCAGGGGTTGTGGATGTGGAGGCCTACGCCATCGAGCGCGCTTATACCCTGCTGGATAACCA
GTTCCCTCCCCAGGAGCAACTGGTGGTGGCGGTCATCGATATCGGCGCCACCATGAGTGCGCTGTCGGTGATGGTCGACG
GTCGTACCGTCTATACCCGCGAGCAGTTGTTTGGCGGTCGCCAGCTCACCGATGAAATCCAGCGTCGCTACGGCCTGTCA
GCGGAAGAGGCCACTCTCGCCAAGCGCCAGGGCGGCAGCGGTCTGCCGGATGACTACTACCCGGAGGTGCTGGAGCCCTT
CCGCGATGCGGTAGTCCAGCAGGTGACACGTTCCCTGCAGTTCTTCTATTCCTCGACTTCCTACAGCGACGTGGATTACA
TCCTCCTCGGCGGCGGTGTCGCCGCCATGGATGGACTCGCCGAGCTGGTGGGTGAGAAGCTGAACAAGCCCACCATGGTG
GCCAATCCTTTCCGGGATATGAGCGTTGCATCCCGTGTCAATCGTCAGGCGCTGGCCAGTGAGGCGCCCTCGCTGATGAT
TGCCGCTGGCCTCGCCATGCGCGAACGGGAGTTTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H8PWW3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.989

98.324

0.511
  comM Acinetobacter nosocomialis M2

51.989

98.324

0.511
  comM Acinetobacter baylyi ADP1

47.727

98.324

0.469
  pilM Legionella pneumophila strain ERS1305867

44.663

99.441

0.444