Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   HUK75_RS24815 Genome accession   NZ_CP054792
Coordinates   5277957..5278829 (+) Length   290 a.a.
NCBI ID   WP_034007824.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SE5431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5262500..5277954 5277957..5278829 flank 3


Gene organization within MGE regions


Location: 5262500..5278829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK75_RS24725 (HUK75_24670) - 5262500..5263720 (+) 1221 WP_062832571.1 tyrosine-type recombinase/integrase -
  HUK75_RS24730 (HUK75_24675) - 5263792..5264613 (-) 822 WP_062832572.1 hypothetical protein -
  HUK75_RS31270 - 5264928..5265053 (+) 126 WP_265324936.1 hypothetical protein -
  HUK75_RS24735 (HUK75_24680) - 5265182..5265343 (+) 162 WP_162998314.1 hypothetical protein -
  HUK75_RS24740 (HUK75_24685) - 5265755..5266069 (+) 315 WP_062832573.1 helix-turn-helix transcriptional regulator -
  HUK75_RS24745 (HUK75_24690) - 5266073..5268472 (+) 2400 WP_079767670.1 bifunctional DNA primase/polymerase -
  HUK75_RS24750 (HUK75_24695) - 5269063..5269578 (+) 516 WP_062832575.1 hypothetical protein -
  HUK75_RS24755 (HUK75_24700) - 5269568..5269816 (+) 249 WP_062832576.1 hypothetical protein -
  HUK75_RS24760 (HUK75_24705) - 5269791..5270237 (+) 447 WP_062832577.1 hypothetical protein -
  HUK75_RS24765 (HUK75_24710) - 5270463..5271179 (+) 717 WP_062832578.1 hypothetical protein -
  HUK75_RS24770 (HUK75_24720) - 5271172..5271447 (+) 276 WP_050714322.1 hypothetical protein -
  HUK75_RS24775 (HUK75_24725) - 5271665..5272132 (+) 468 WP_062832579.1 hypothetical protein -
  HUK75_RS24780 (HUK75_24730) - 5272126..5272506 (+) 381 WP_062832580.1 hypothetical protein -
  HUK75_RS24785 (HUK75_24735) - 5272597..5273064 (-) 468 WP_062832581.1 hypothetical protein -
  HUK75_RS24795 (HUK75_24745) - 5273498..5274196 (-) 699 WP_016254081.1 hypothetical protein -
  HUK75_RS24800 (HUK75_24750) - 5274259..5274801 (-) 543 WP_235179930.1 pilin -
  HUK75_RS24805 (HUK75_24755) pilB 5275030..5276733 (+) 1704 WP_003141354.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HUK75_RS24810 (HUK75_24760) pilC 5276737..5277954 (+) 1218 WP_016254080.1 type II secretion system F family protein Machinery gene
  HUK75_RS24815 (HUK75_24765) pilD 5277957..5278829 (+) 873 WP_034007824.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31825.82 Da        Isoelectric Point: 7.0816

>NTDB_id=454672 HUK75_RS24815 WP_034007824.1 5277957..5278829(+) (pilD) [Pseudomonas aeruginosa strain SE5431]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPSSACPHCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=454672 HUK75_RS24815 WP_034007824.1 5277957..5278829(+) (pilD) [Pseudomonas aeruginosa strain SE5431]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCAGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAGTTCGGCGTGCCCGCACTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.363

99.655

0.552
  pilD Vibrio campbellii strain DS40M4

53.497

98.621

0.528
  pilD Acinetobacter nosocomialis M2

50.352

97.931

0.493
  pilD Acinetobacter baumannii D1279779

50.352

97.931

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472