Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   I33_RS12350 Genome accession   NC_017195
Coordinates   2488141..2488758 (-) Length   205 a.a.
NCBI ID   WP_014477388.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. RO-NN-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2483141..2493758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I33_RS12325 (I33_2639) rpsT 2483443..2483709 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  I33_RS12330 (I33_2640) holA 2483724..2484767 (-) 1044 WP_014477385.1 DNA polymerase III subunit delta -
  I33_RS20990 (I33_2641) - 2484807..2484956 (-) 150 WP_003229985.1 hypothetical protein -
  I33_RS20480 yqzM 2484997..2485131 (+) 135 WP_003229983.1 YqzM family protein -
  I33_RS12340 (I33_2643) comEC 2485171..2487501 (-) 2331 WP_041519416.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  I33_RS12345 (I33_2645) comEB 2487505..2488074 (-) 570 WP_014477387.1 ComE operon protein 2 -
  I33_RS12350 (I33_2646) comEA 2488141..2488758 (-) 618 WP_014477388.1 competence protein ComEA Machinery gene
  I33_RS12355 (I33_2647) comER 2488842..2489663 (+) 822 WP_014477389.1 late competence protein ComER -
  I33_RS12360 (I33_2648) yqeM 2489729..2490472 (-) 744 WP_014477390.1 class I SAM-dependent methyltransferase -
  I33_RS12365 (I33_2649) rsfS 2490469..2490825 (-) 357 WP_014477391.1 ribosome silencing factor -
  I33_RS12370 (I33_2650) yqeK 2490843..2491403 (-) 561 WP_014477392.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  I33_RS12375 (I33_2651) nadD 2491393..2491962 (-) 570 WP_014477393.1 nicotinate-nucleotide adenylyltransferase -
  I33_RS12380 (I33_2652) yhbY 2491974..2492264 (-) 291 WP_014477394.1 ribosome assembly RNA-binding protein YhbY -
  I33_RS12385 (I33_2653) aroE 2492258..2493100 (-) 843 WP_014477395.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21894.67 Da        Isoelectric Point: 4.7354

>NTDB_id=45425 I33_RS12350 WP_014477388.1 2488141..2488758(-) (comEA) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGAGGSVQSDGGKGALVNINTATLEELQDISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=45425 I33_RS12350 WP_014477388.1 2488141..2488758(-) (comEA) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTGGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTAGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGCCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAATTACAAGACATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGACGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment