Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HR116_RS02015 Genome accession   NZ_CP054591
Coordinates   452658..453722 (+) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CDN118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447658..458722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR116_RS02005 - 448632..449900 (+) 1269 WP_003103278.1 malic enzyme -
  HR116_RS02010 - 450005..452473 (-) 2469 WP_003114576.1 penicillin-binding protein 1A -
  HR116_RS02015 pilM 452658..453722 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  HR116_RS02020 pilN 453722..454318 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  HR116_RS02025 pilO 454315..454938 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  HR116_RS02030 pilP 454935..455459 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  HR116_RS02035 pilQ 455513..457657 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  HR116_RS02040 aroK 457669..458187 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=453754 HR116_RS02015 WP_003110888.1 452658..453722(+) (pilM) [Pseudomonas aeruginosa strain CDN118]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=453754 HR116_RS02015 WP_003110888.1 452658..453722(+) (pilM) [Pseudomonas aeruginosa strain CDN118]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466