Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   HT135_RS13840 Genome accession   NZ_CP054584
Coordinates   2691850..2692467 (-) Length   205 a.a.
NCBI ID   WP_106020123.1    Uniprot ID   -
Organism   Bacillus halotolerans strain KKD1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2686850..2697467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT135_RS13810 (HT135_13805) rpsT 2687153..2687419 (+) 267 WP_010335003.1 30S ribosomal protein S20 -
  HT135_RS13815 (HT135_13810) holA 2687434..2688477 (-) 1044 WP_106020122.1 DNA polymerase III subunit delta -
  HT135_RS13820 (HT135_13815) - 2688517..2688666 (-) 150 WP_003229985.1 hypothetical protein -
  HT135_RS13825 (HT135_13820) - 2688707..2688841 (+) 135 WP_010335005.1 YqzM family protein -
  HT135_RS13830 (HT135_13825) comEC 2688880..2691210 (-) 2331 WP_106020344.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  HT135_RS13835 (HT135_13830) - 2691214..2691783 (-) 570 WP_010335007.1 ComE operon protein 2 -
  HT135_RS13840 (HT135_13835) comEA 2691850..2692467 (-) 618 WP_106020123.1 helix-hairpin-helix domain-containing protein Machinery gene
  HT135_RS13845 (HT135_13840) comER 2692551..2693372 (+) 822 WP_024122113.1 late competence protein ComER -
  HT135_RS13850 (HT135_13845) - 2693437..2694180 (-) 744 WP_106020124.1 class I SAM-dependent DNA methyltransferase -
  HT135_RS13855 (HT135_13850) rsfS 2694177..2694533 (-) 357 WP_024122115.1 ribosome silencing factor -
  HT135_RS13860 (HT135_13855) yqeK 2694551..2695111 (-) 561 WP_106020125.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  HT135_RS13865 (HT135_13860) - 2695101..2695670 (-) 570 WP_010335014.1 nicotinate-nucleotide adenylyltransferase -
  HT135_RS13870 (HT135_13865) yhbY 2695681..2695971 (-) 291 WP_106020126.1 ribosome assembly RNA-binding protein YhbY -
  HT135_RS13875 (HT135_13870) aroE 2695971..2696807 (-) 837 WP_038954228.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21924.71 Da        Isoelectric Point: 4.9829

>NTDB_id=453728 HT135_RS13840 WP_106020123.1 2691850..2692467(-) (comEA) [Bacillus halotolerans strain KKD1]
MNWLNQHKTIIMSAGAAAVLIAVLCQFFFSGHNKEPVKQVISSEKGNAEVKQEATKDKAEENIVIDIKGAVKHPGVYEMH
TGDRVSQAIEKAGGIGEKADETQVNLAELLQDGTVVYIPSEGEEAAQQAAGGAVQSSKGKETLVNINTATLEDLQNISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=453728 HT135_RS13840 WP_106020123.1 2691850..2692467(-) (comEA) [Bacillus halotolerans strain KKD1]
TTGAATTGGTTGAATCAACATAAAACGATAATCATGTCAGCGGGCGCTGCTGCTGTTCTCATTGCAGTTCTTTGCCAGTT
TTTTTTCAGCGGACATAACAAAGAGCCTGTAAAACAAGTTATATCTAGTGAAAAGGGAAATGCGGAGGTCAAGCAGGAAG
CAACAAAAGACAAAGCTGAAGAAAATATTGTAATTGATATAAAAGGCGCTGTTAAGCATCCCGGCGTTTATGAAATGCAT
ACGGGAGACAGAGTATCGCAGGCTATTGAGAAAGCAGGTGGCATTGGTGAAAAAGCAGATGAAACGCAAGTGAATTTGGC
GGAACTTTTACAGGACGGTACAGTGGTGTACATACCGAGTGAGGGAGAAGAGGCGGCGCAGCAAGCGGCCGGCGGCGCTG
TACAAAGCAGTAAGGGGAAGGAGACATTGGTCAATATCAACACAGCAACATTAGAGGATTTACAAAACATTTCGGGAGTA
GGACCTTCAAAAGCCGAAGCGATTATTGCGTATCGGGAAGAGAACGGGCGTTTCCAGACAATCGAAGATATCACAAAGGT
TTCGGGAATTGGCGAAAAATCTTTTGAAAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(64-119)

Helix-hairpin-helix motif (HHH_3)

(141-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

75.728

100

0.761
  comEA Staphylococcus aureus MW2

37.727

100

0.405
  comEA Staphylococcus aureus N315

36.486

100

0.395