Detailed information NTG0453576

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FOC67_RS23970
• Genome accession: NZ_CP054572
• Coordinates: 5147955..5149103 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas sp. FDAARGOS_761
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5142955..5154103

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC67_RS23950 (FOC67_23950)	-	5143693..5144877 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
FOC67_RS23955 (FOC67_23955)	-	5144874..5146247 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
FOC67_RS23960 (FOC67_23960)	-	5146490..5146894 (-)	405	WP_003084560.1	NINE protein	-
FOC67_RS23965 (FOC67_23965)	czcD	5147048..5147947 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
FOC67_RS23970 (FOC67_23970)	pilU	5147955..5149103 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
FOC67_RS23975 (FOC67_23975)	pilT	5149281..5150315 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
FOC67_RS23980 (FOC67_23980)	-	5150530..5151222 (+)	693	WP_009315059.1	YggS family pyridoxal phosphate-dependent enzyme	-
FOC67_RS23985 (FOC67_23985)	proC	5151234..5152055 (+)	822	WP_009315058.1	pyrroline-5-carboxylate reductase	-
FOC67_RS23990 (FOC67_23990)	-	5152066..5152659 (+)	594	WP_003084544.1	YggT family protein	-
FOC67_RS23995 (FOC67_23995)	-	5152656..5152952 (+)	297	WP_031627661.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=453576 FOC67_RS23970 WP_003110016.1 5147955..5149103(-) (pilU) [Pseudomonas sp. FDAARGOS_761]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=453576 FOC67_RS23970 WP_003110016.1 5147955..5149103(-) (pilU) [Pseudomonas sp. FDAARGOS_761]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382