Detailed information NTG0452881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6H93_RS09685
• Genome accession: NZ_CP066016
• Coordinates: 2020295..2021413 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain FDAARGOS_1036
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2015295..2026413

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6H93_RS09675 (I6H93_09675)	-	2018411..2019103 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6H93_RS09680 (I6H93_09680)	pilT	2019230..2020267 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
I6H93_RS09685 (I6H93_09685)	pilU	2020295..2021413 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6H93_RS09690 (I6H93_09690)	fur	2021483..2021920 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
I6H93_RS09695 (I6H93_09695)	-	2022032..2022430 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
I6H93_RS09700 (I6H93_09700)	-	2022485..2022808 (-)	324	WP_000442585.1	RnfH family protein	-
I6H93_RS09705 (I6H93_09705)	-	2022808..2023875 (-)	1068	WP_000092403.1	hypothetical protein	-
I6H93_RS09710 (I6H93_09710)	-	2024028..2024483 (-)	456	WP_000782976.1	bacteriohemerythrin	-
I6H93_RS09715 (I6H93_09715)	-	2024736..2025584 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=452881 I6H93_RS09685 WP_000347036.1 2020295..2021413(+) (pilU) [Acinetobacter baumannii strain FDAARGOS_1036]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=452881 I6H93_RS09685 WP_000347036.1 2020295..2021413(+) (pilU) [Acinetobacter baumannii strain FDAARGOS_1036]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392