Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   HT132_RS03730 Genome accession   NZ_CP054479
Coordinates   704281..705666 (+) Length   461 a.a.
NCBI ID   WP_174532388.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699281..710666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS03710 (HT132_03710) - 700428..701075 (-) 648 WP_007407485.1 YigZ family protein -
  HT132_RS03715 (HT132_03715) degS 701298..702461 (+) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  HT132_RS03720 (HT132_03720) degU 702538..703227 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  HT132_RS03725 (HT132_03725) - 703325..704173 (+) 849 WP_003151387.1 DegV family protein -
  HT132_RS03730 (HT132_03730) comFA 704281..705666 (+) 1386 WP_174532388.1 DEAD/DEAH box helicase Machinery gene
  HT132_RS03735 (HT132_03735) - 705724..706008 (+) 285 WP_045209016.1 competence protein ComFB -
  HT132_RS03740 (HT132_03740) comFC 706014..706703 (+) 690 WP_202729384.1 phosphoribosyltransferase family protein Machinery gene
  HT132_RS03745 (HT132_03745) - 706777..707196 (+) 420 WP_061861586.1 TIGR03826 family flagellar region protein -
  HT132_RS03750 (HT132_03750) flgM 707266..707532 (+) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  HT132_RS03755 (HT132_03755) - 707547..708029 (+) 483 WP_003151394.1 flagellar protein FlgN -
  HT132_RS03760 (HT132_03760) flgK 708045..709562 (+) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  HT132_RS03765 (HT132_03765) flgL 709574..710491 (+) 918 WP_014419007.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52509.85 Da        Isoelectric Point: 10.2278

>NTDB_id=452754 HT132_RS03730 WP_174532388.1 704281..705666(+) (comFA) [Bacillus amyloliquefaciens strain R8-25]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQTPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKEGQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSVHVLKKTTDYFQKLNVRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=452754 HT132_RS03730 WP_174532388.1 704281..705666(+) (comFA) [Bacillus amyloliquefaciens strain R8-25]
ATGTCCGAGGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
CTCAGACCAGCGTATCAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAACCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGGGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCAGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTACTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAAGACAAAGGCCGCCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTTCCGG
AGCCGCGTTTTTTGTGGTGCGGAAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGTCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACGTACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACCGAGAGCGCGCTCGTGCAGATCGCAGGCAGAACGGGAAGGCACTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAGCATATGAACAAATTGG
CACAAAAAAGTAAATTGCTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(328-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.609

99.783

0.625