NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	HSX12_RS12105	Genome accession	NZ_CP054467
Coordinates	2489606..2490676 (-)	Length	356 a.a.
NCBI ID	WP_003153083.1	Uniprot ID	-
Organism	Bacillus velezensis strain CF57
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2484606..2495676

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSX12_RS12055 (HSX12_11945)	sipW	2484666..2485250 (-)	585	WP_015240205.1	signal peptidase I SipW	-
HSX12_RS12060 (HSX12_11950)	tapA	2485222..2485893 (-)	672	WP_015417813.1	amyloid fiber anchoring/assembly protein TapA	-
HSX12_RS12065 (HSX12_11955)	-	2486152..2486481 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
HSX12_RS12070 (HSX12_11960)	-	2486521..2486700 (-)	180	WP_003153093.1	YqzE family protein	-
HSX12_RS12075 (HSX12_11965)	comGG	2486757..2487134 (-)	378	WP_015417814.1	competence type IV pilus minor pilin ComGG	Machinery gene
HSX12_RS12080 (HSX12_11970)	comGF	2487135..2487635 (-)	501	WP_258548905.1	competence type IV pilus minor pilin ComGF	-
HSX12_RS12085 (HSX12_11975)	comGE	2487544..2487858 (-)	315	WP_031378943.1	competence type IV pilus minor pilin ComGE	-
HSX12_RS12090 (HSX12_11980)	comGD	2487842..2488279 (-)	438	WP_015417817.1	competence type IV pilus minor pilin ComGD	Machinery gene
HSX12_RS12095 (HSX12_11985)	comGC	2488269..2488577 (-)	309	WP_015417818.1	competence type IV pilus major pilin ComGC	Machinery gene
HSX12_RS12100 (HSX12_11990)	comGB	2488582..2489619 (-)	1038	WP_015417819.1	competence type IV pilus assembly protein ComGB	Machinery gene
HSX12_RS12105 (HSX12_11995)	comGA	2489606..2490676 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
HSX12_RS12110 (HSX12_12000)	-	2490869..2491819 (-)	951	WP_015417820.1	magnesium transporter CorA family protein	-
HSX12_RS12115 (HSX12_12005)	-	2491965..2493266 (+)	1302	WP_012117986.1	hemolysin family protein	-
HSX12_RS12120 (HSX12_12010)	-	2493304..2494134 (-)	831	WP_015417821.1	STAS domain-containing protein	-
HSX12_RS12130 (HSX12_12020)	-	2494341..2494721 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
HSX12_RS12135 (HSX12_12025)	-	2494958..2495203 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.85 Da Isoelectric Point: 9.4729

                                >NTDB_id=452628 HSX12_RS12105 WP_003153083.1 2489606..2490676(-) (comGA) [Bacillus velezensis strain CF57]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=452628 HSX12_RS12105 WP_003153083.1 2489606..2490676(-) (comGA) [Bacillus velezensis strain CF57]

GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0452628