Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   HSX12_RS12100 Genome accession   NZ_CP054467
Coordinates   2488582..2489619 (-) Length   345 a.a.
NCBI ID   WP_015417819.1    Uniprot ID   -
Organism   Bacillus velezensis strain CF57     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2483582..2494619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSX12_RS12050 (HSX12_11940) tasA 2483816..2484601 (-) 786 WP_007408329.1 biofilm matrix protein TasA -
  HSX12_RS12055 (HSX12_11945) sipW 2484666..2485250 (-) 585 WP_015240205.1 signal peptidase I SipW -
  HSX12_RS12060 (HSX12_11950) tapA 2485222..2485893 (-) 672 WP_015417813.1 amyloid fiber anchoring/assembly protein TapA -
  HSX12_RS12065 (HSX12_11955) - 2486152..2486481 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  HSX12_RS12070 (HSX12_11960) - 2486521..2486700 (-) 180 WP_003153093.1 YqzE family protein -
  HSX12_RS12075 (HSX12_11965) comGG 2486757..2487134 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  HSX12_RS12080 (HSX12_11970) comGF 2487135..2487635 (-) 501 WP_258548905.1 competence type IV pilus minor pilin ComGF -
  HSX12_RS12085 (HSX12_11975) comGE 2487544..2487858 (-) 315 WP_031378943.1 competence type IV pilus minor pilin ComGE -
  HSX12_RS12090 (HSX12_11980) comGD 2487842..2488279 (-) 438 WP_015417817.1 competence type IV pilus minor pilin ComGD Machinery gene
  HSX12_RS12095 (HSX12_11985) comGC 2488269..2488577 (-) 309 WP_015417818.1 competence type IV pilus major pilin ComGC Machinery gene
  HSX12_RS12100 (HSX12_11990) comGB 2488582..2489619 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  HSX12_RS12105 (HSX12_11995) comGA 2489606..2490676 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  HSX12_RS12110 (HSX12_12000) - 2490869..2491819 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  HSX12_RS12115 (HSX12_12005) - 2491965..2493266 (+) 1302 WP_012117986.1 hemolysin family protein -
  HSX12_RS12120 (HSX12_12010) - 2493304..2494134 (-) 831 WP_015417821.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39689.06 Da        Isoelectric Point: 9.5935

>NTDB_id=452627 HSX12_RS12100 WP_015417819.1 2488582..2489619(-) (comGB) [Bacillus velezensis strain CF57]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=452627 HSX12_RS12100 WP_015417819.1 2488582..2489619(-) (comGB) [Bacillus velezensis strain CF57]
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAGTCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGAGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614