Detailed information NTG0452404

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JBL37_RS26985
• Genome accession: NZ_CP065948
• Coordinates: 5748970..5750118 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PAM68
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5743970..5755118

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JBL37_RS26960 (JBL37_26965)	-	5745121..5745417 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
JBL37_RS26965 (JBL37_26970)	-	5745414..5746007 (-)	594	WP_003084544.1	YggT family protein	-
JBL37_RS26970 (JBL37_26975)	proC	5746018..5746839 (-)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
JBL37_RS26975 (JBL37_26980)	-	5746851..5747543 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
JBL37_RS26980 (JBL37_26985)	pilT	5747758..5748792 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JBL37_RS26985 (JBL37_26990)	pilU	5748970..5750118 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
JBL37_RS26990 (JBL37_26995)	czcD	5750126..5751025 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
JBL37_RS26995 (JBL37_27000)	-	5751179..5751583 (+)	405	WP_003084560.1	NINE protein	-
JBL37_RS27000 (JBL37_27005)	-	5751826..5753199 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
JBL37_RS27005 (JBL37_27010)	-	5753196..5754380 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=452404 JBL37_RS26985 WP_003110016.1 5748970..5750118(+) (pilU) [Pseudomonas aeruginosa strain PAM68]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=452404 JBL37_RS26985 WP_003110016.1 5748970..5750118(+) (pilU) [Pseudomonas aeruginosa strain PAM68]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382