Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HTY60_RS12635 Genome accession   NZ_CP054415
Coordinates   2451207..2452277 (-) Length   356 a.a.
NCBI ID   WP_013352872.1    Uniprot ID   A0A9P1JI17
Organism   Bacillus amyloliquefaciens strain 205     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2446207..2457277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HTY60_RS12585 (HTY60_12585) sipW 2446269..2446853 (-) 585 WP_013352863.1 signal peptidase I SipW -
  HTY60_RS12590 (HTY60_12590) tapA 2446825..2447496 (-) 672 WP_013352864.1 amyloid fiber anchoring/assembly protein TapA -
  HTY60_RS12595 (HTY60_12595) - 2447754..2448083 (+) 330 WP_013352865.1 DUF3889 domain-containing protein -
  HTY60_RS12600 (HTY60_12600) - 2448124..2448303 (-) 180 WP_013352866.1 YqzE family protein -
  HTY60_RS12605 (HTY60_12605) comGG 2448357..2448734 (-) 378 WP_013352867.1 competence type IV pilus minor pilin ComGG Machinery gene
  HTY60_RS12610 (HTY60_12610) comGF 2448736..2449236 (-) 501 WP_013352868.1 competence type IV pilus minor pilin ComGF -
  HTY60_RS12615 (HTY60_12615) comGE 2449145..2449459 (-) 315 WP_014470662.1 competence type IV pilus minor pilin ComGE Machinery gene
  HTY60_RS12620 (HTY60_12620) comGD 2449443..2449880 (-) 438 WP_013352869.1 competence type IV pilus minor pilin ComGD Machinery gene
  HTY60_RS12625 (HTY60_12625) comGC 2449870..2450178 (-) 309 WP_013352870.1 competence type IV pilus major pilin ComGC Machinery gene
  HTY60_RS12630 (HTY60_12630) comGB 2450183..2451220 (-) 1038 WP_013352871.1 competence type IV pilus assembly protein ComGB Machinery gene
  HTY60_RS12635 (HTY60_12635) comGA 2451207..2452277 (-) 1071 WP_013352872.1 competence type IV pilus ATPase ComGA Machinery gene
  HTY60_RS12640 (HTY60_12640) - 2452471..2453421 (-) 951 WP_013352873.1 magnesium transporter CorA family protein -
  HTY60_RS12645 (HTY60_12645) - 2453568..2454869 (+) 1302 WP_013352874.1 hemolysin family protein -
  HTY60_RS12650 (HTY60_12650) - 2454907..2455734 (-) 828 WP_013352875.1 STAS domain-containing protein -
  HTY60_RS12660 (HTY60_12660) - 2455947..2456327 (-) 381 WP_013352876.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  HTY60_RS12665 (HTY60_12665) - 2456563..2456808 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40430.67 Da        Isoelectric Point: 9.0345

>NTDB_id=452320 HTY60_RS12635 WP_013352872.1 2451207..2452277(-) (comGA) [Bacillus amyloliquefaciens strain 205]
MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKTDNCSVYCSLYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=452320 HTY60_RS12635 WP_013352872.1 2451207..2452277(-) (comGA) [Bacillus amyloliquefaciens strain 205]
ATGGAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC
GAGGGAAAGGGATGCTTTCATTCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAATTTTTATCAGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT
CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
ATTGCCTAAAAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACCACCCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCACCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACAGGACACCTCGTACTGTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCAAAACGGACAACTGTTCCGTGTACTGCAGTCTATACA
GGCAGACACGCCGTGCTGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAAGAAGCAAAAGGGGAG
CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGCATCGCGCTCGGTTATGTCACGACTGATAATTA
TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376
  pilF Thermus thermophilus HB27

38

98.315

0.374