Detailed information NTG0452237

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: JBL36_RS07625
• Genome accession: NZ_CP065947
• Coordinates: 1613887..1615035 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain PAS6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1608887..1620035

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JBL36_RS07605 (JBL36_07605)	-	1609625..1610809 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
JBL36_RS07610 (JBL36_07610)	-	1610806..1612179 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
JBL36_RS07615 (JBL36_07615)	-	1612422..1612826 (-)	405	WP_003084560.1	NINE protein	-
JBL36_RS07620 (JBL36_07620)	czcD	1612980..1613879 (+)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
JBL36_RS07625 (JBL36_07625)	pilU	1613887..1615035 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
JBL36_RS07630 (JBL36_07630)	pilT	1615213..1616247 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
JBL36_RS07635 (JBL36_07635)	-	1616462..1617154 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
JBL36_RS07640 (JBL36_07640)	proC	1617166..1617987 (+)	822	WP_023081631.1	pyrroline-5-carboxylate reductase	-
JBL36_RS07645 (JBL36_07645)	-	1617998..1618591 (+)	594	WP_003084544.1	YggT family protein	-
JBL36_RS07650 (JBL36_07650)	-	1618588..1618884 (+)	297	WP_031628073.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=452237 JBL36_RS07625 WP_003110016.1 1613887..1615035(-) (pilU) [Pseudomonas aeruginosa strain PAS6]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=452237 JBL36_RS07625 WP_003110016.1 1613887..1615035(-) (pilU) [Pseudomonas aeruginosa strain PAS6]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382