Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IAU57_RS06245 Genome accession   NZ_CP065848
Coordinates   1327383..1327982 (+) Length   199 a.a.
NCBI ID   WP_126550799.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CMC-097     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1322383..1332982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IAU57_RS06230 (IAU57_06230) - 1323070..1325541 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  IAU57_RS06235 (IAU57_06235) pilM 1325726..1326790 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IAU57_RS06240 (IAU57_06240) pilN 1326790..1327386 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  IAU57_RS06245 (IAU57_06245) pilO 1327383..1327982 (+) 600 WP_126550799.1 type 4a pilus biogenesis protein PilO Machinery gene
  IAU57_RS06250 (IAU57_06250) comL/pilP 1327979..1328503 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  IAU57_RS06255 (IAU57_06255) pilQ 1328557..1330701 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  IAU57_RS06260 (IAU57_06260) aroK 1330713..1331231 (+) 519 WP_003095833.1 shikimate kinase AroK -
  IAU57_RS06265 (IAU57_06265) aroB 1331281..1332387 (+) 1107 WP_003107839.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22065.36 Da        Isoelectric Point: 4.6641

>NTDB_id=451513 IAU57_RS06245 WP_126550799.1 1327383..1327982(+) (pilO) [Pseudomonas aeruginosa strain CMC-097]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPRMSSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 600 bp        

>NTDB_id=451513 IAU57_RS06245 WP_126550799.1 1327383..1327982(+) (pilO) [Pseudomonas aeruginosa strain CMC-097]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGCGCATGAGCAGCATCCTGGC
CAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

44.041

96.985

0.427
  pilO Acinetobacter baumannii D1279779

42.632

95.477

0.407
  comO/pilO Acinetobacter baylyi ADP1

42.857

94.975

0.407