Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   G4D73_RS02690 Genome accession   NZ_CP053947
Coordinates   586140..587336 (-) Length   398 a.a.
NCBI ID   WP_005251952.1    Uniprot ID   N9PSE4
Organism   Acinetobacter sp. WY4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 587932..596235 586140..587336 flank 596


Gene organization within MGE regions


Location: 586140..596235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4D73_RS02690 pilU 586140..587336 (-) 1197 WP_005251952.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  G4D73_RS02695 tet(39) 587932..589119 (-) 1188 WP_087551687.1 tetracycline efflux MFS transporter Tet(39) -
  G4D73_RS02700 - 589185..589826 (+) 642 WP_004787586.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
  G4D73_RS02705 - 589985..590305 (+) 321 WP_000897307.1 type II toxin-antitoxin system RelE/ParE family toxin -
  G4D73_RS02710 nadS 590298..590570 (+) 273 WP_000369781.1 NadS family protein -
  G4D73_RS02715 msr(E) 591063..592538 (+) 1476 WP_000052512.1 ABC-F type ribosomal protection protein Msr(E) -
  G4D73_RS02720 - 592594..593478 (+) 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -
  G4D73_RS02725 - 593668..594279 (-) 612 WP_015060246.1 recombinase family protein -
  G4D73_RS02730 - 594480..594755 (+) 276 WP_004967304.1 DUF6966 domain-containing protein -
  G4D73_RS02735 - 594901..596235 (+) 1335 WP_213687328.1 ISNCY-like element ISAba32 family transposase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45112.72 Da        Isoelectric Point: 6.6224

>NTDB_id=449287 G4D73_RS02690 WP_005251952.1 586140..587336(-) (pilU) [Acinetobacter sp. WY4]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAITVPGVSRFRVNVFVQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLMSDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGEISYEEALRNADSTNELRLQIKLKSNRQDTTVATEATQFTMMQEPKAEEKQAENTSVSE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=449287 G4D73_RS02690 WP_005251952.1 586140..587336(-) (pilU) [Acinetobacter sp. WY4]
ATGTTCACGGCAGAATTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACTAAAGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCGAGTATTAAACATCAAGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CAGCTGAAAAAACCAAGCTGTTTGCCTACAGTCTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTTGAGTGC
AATTTTGCCATCACCGTGCCAGGTGTTTCCCGTTTTCGGGTCAATGTGTTTGTACAACAACTTAATGTTGGCATGGTGAT
TCGTACCATTACTGCAGAAATTCCAAATTTCCAGAAGCTGAAATTACCGGAATCTCTGAAACATGTGATTATGGAAAAAC
GTGGACTGGTGCTGGTGGTCGGAGGAACAGGTTCCGGTAAATCAACTTCACTCGCAGCCATGATTGACTATCGAAATGAA
AATTCAGCCGGGCATATCATTACCGTAGAAGATCCGGTTGAATATGTGCACAAGCATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCCTGGCATAATGCGCTGAAAAATACCTTGCGTCAGGCACCAGATGTGATTCTGATCG
GCGAAATTCGCGATACTGAAACCATGGAACACGCGATTGCTTTTGCTGAAACTGGACATCTGTGTCTGGGAACGCTGCAC
TCGAACAATGCCAACCAGACTTTGGACCGGATTATCAACTTCTTCCCGGAAGAACGCCGGAACCAGCTATTAATGGATTT
GTCATCCAATATGAAGGCGATCATTTCACAGCGTCTGATTCGTACTCAAGATGGTAAAGGCCGTCGTGCAGCGATTGAAA
TCATGTTAAATACGCCACTGATGTCTGACCTGATTTTGAAAGGTGAATTCCACGAATTGAAAGCGATTATGGCGAAATCG
CGTGAACTGGGCATGCAGACCTTTGACCAGGCCTTATTTGACCTGTATAACCAGGGTGAAATTTCTTATGAAGAAGCCTT
GCGCAATGCCGATTCGACCAATGAACTGCGCTTACAAATCAAGCTGAAAAGTAACCGACAGGACACTACCGTAGCCACAG
AGGCAACCCAATTCACCATGATGCAAGAGCCAAAAGCGGAAGAGAAACAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9PSE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.494

87.437

0.538
  pilU Acinetobacter baylyi ADP1

55.908

87.186

0.487
  pilU Vibrio cholerae strain A1552

52.394

89.196

0.467
  pilT Pseudomonas aeruginosa PAK

44.172

81.91

0.362