Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CHLWT_RS06400 Genome accession   NZ_CP053828
Coordinates   1256636..1257703 (-) Length   355 a.a.
NCBI ID   WP_063997948.1    Uniprot ID   A0A562XC04
Organism   Campylobacter hyointestinalis subsp. lawsonii strain CHY5     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1251636..1262703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHLWT_RS06375 (CHLWT_1248) - 1252285..1252827 (-) 543 WP_063997952.1 DUF1882 domain-containing protein -
  CHLWT_RS06380 (CHLWT_1249) - 1252827..1254071 (-) 1245 WP_063997951.1 serine hydroxymethyltransferase -
  CHLWT_RS06385 (CHLWT_1250) lysS 1254073..1255578 (-) 1506 WP_244948813.1 lysine--tRNA ligase -
  CHLWT_RS06390 (CHLWT_1251) - 1255582..1256064 (-) 483 WP_176320883.1 Fur family transcriptional regulator -
  CHLWT_RS06395 (CHLWT_1252) - 1256067..1256636 (-) 570 WP_244948767.1 CvpA family protein -
  CHLWT_RS06400 (CHLWT_1253) pilT 1256636..1257703 (-) 1068 WP_063997948.1 type IV pilus twitching motility protein PilT Machinery gene
  CHLWT_RS06405 (CHLWT_1254) gatC 1257708..1257995 (-) 288 WP_112000716.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  CHLWT_RS06410 (CHLWT_1255) - 1258203..1258763 (+) 561 WP_112000717.1 hypothetical protein -
  CHLWT_RS06415 (CHLWT_1256) - 1258768..1259766 (+) 999 WP_063997945.1 hypothetical protein -
  CHLWT_RS06420 (CHLWT_1257) - 1259768..1260094 (+) 327 WP_112000718.1 hypothetical protein -
  CHLWT_RS06425 (CHLWT_1258) - 1260081..1260713 (+) 633 WP_111948350.1 type III pantothenate kinase -
  CHLWT_RS06430 (CHLWT_1259) hisG 1260701..1261339 (+) 639 WP_112000719.1 ATP phosphoribosyltransferase -
  CHLWT_RS06435 (CHLWT_1260) - 1261518..1262618 (+) 1101 WP_244948768.1 metallophosphoesterase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 39428.25 Da        Isoelectric Point: 6.8314

>NTDB_id=447971 CHLWT_RS06400 WP_063997948.1 1256636..1257703(-) (pilT) [Campylobacter hyointestinalis subsp. lawsonii strain CHY5]
MSINIEKLLKTLAYNKGSDLHLVARSEPQIRISGVLRPLQCGILDGDEVENLCYTVLTDSQKSKLEENKELDFSIELPGI
GRFRGNCYYTMNGSLAAAFRMIPLEIPSLDDLKAPEIFKDIVKREKGLVLVTGPTGSGKSTTLAAMLNEINLTERKHIIT
IEDPIEFVHKNKKSLFSHRNIGTDTKTFASALKYALREDPDIILVGDLRDIEAISIAISAAETGHLVFGTLHTNSAIQTI
NRIVDSFDESEQSQIRNMLSISLHAVISQALVPLTSGGRIAIHEILINNPAISNLIRENKIHQIYSQLQLNQQNTGMQTQ
TQDMERAFKSGLITKDNALRYSTNKQELIGKIGHL

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=447971 CHLWT_RS06400 WP_063997948.1 1256636..1257703(-) (pilT) [Campylobacter hyointestinalis subsp. lawsonii strain CHY5]
ATGAGTATAAATATAGAAAAGCTTCTAAAAACCTTGGCTTATAACAAAGGTAGTGATTTACATCTAGTCGCTAGAAGCGA
ACCTCAGATCCGTATAAGTGGAGTTTTGAGACCTTTGCAGTGCGGAATTTTAGATGGAGATGAAGTAGAAAATTTATGTT
ATACGGTGCTTACGGACTCACAAAAAAGCAAACTAGAAGAAAATAAAGAATTAGACTTTTCTATAGAGTTGCCAGGCATA
GGTCGTTTTAGGGGAAATTGCTATTATACTATGAATGGTAGTTTGGCTGCGGCTTTTCGTATGATTCCTTTAGAAATCCC
AAGTTTAGATGATCTAAAAGCTCCTGAGATATTTAAGGATATAGTAAAACGAGAAAAAGGGCTTGTTTTGGTCACAGGAC
CTACTGGTAGTGGCAAATCAACTACTTTAGCAGCTATGTTAAATGAGATAAACTTAACTGAGAGAAAGCATATCATTACT
ATAGAAGATCCTATAGAATTTGTTCATAAAAATAAAAAATCTCTCTTTTCTCATAGAAATATAGGAACCGATACAAAGAC
ATTTGCTAGTGCCTTAAAGTATGCTCTTAGGGAAGATCCAGATATTATATTAGTAGGCGATCTGCGTGATATAGAAGCTA
TATCTATAGCCATATCTGCAGCAGAAACAGGGCATTTGGTATTTGGCACTTTGCATACAAACTCAGCCATACAGACTATA
AATAGAATAGTAGATAGTTTTGATGAGAGTGAGCAATCTCAGATAAGAAATATGCTTAGTATATCTTTGCATGCTGTCAT
ATCTCAAGCACTAGTTCCATTAACTAGCGGTGGTAGGATAGCCATACATGAGATACTTATCAACAATCCTGCTATATCAA
ATTTAATAAGAGAGAATAAAATACATCAAATTTATTCTCAGTTGCAGTTAAATCAGCAAAATACCGGTATGCAAACGCAA
ACTCAAGATATGGAAAGAGCATTTAAATCAGGGCTTATAACAAAAGATAATGCCCTTAGATACTCGACAAATAAACAAGA
ATTAATAGGAAAGATAGGGCATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A562XC04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

52.738

97.746

0.515
  pilT Neisseria meningitidis 8013

52.45

97.746

0.513
  pilT Vibrio cholerae strain A1552

52.568

93.239

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.568

93.239

0.49
  pilT Legionella pneumophila strain Lp02

50.437

96.62

0.487
  pilT Legionella pneumophila strain ERS1305867

50.437

96.62

0.487
  pilT Pseudomonas stutzeri DSM 10701

50.893

94.648

0.482
  pilT Pseudomonas aeruginosa PAK

50.298

94.648

0.476
  pilT Acinetobacter nosocomialis M2

50.298

94.648

0.476
  pilT Acinetobacter baumannii D1279779

50.298

94.648

0.476
  pilT Acinetobacter baumannii strain A118

50.298

94.648

0.476
  pilT Acinetobacter baylyi ADP1

51.057

93.239

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

98.028

0.434
  pilU Acinetobacter baylyi ADP1

38.506

98.028

0.377
  pilU Pseudomonas stutzeri DSM 10701

37.853

99.718

0.377