Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HPY06_RS01330 Genome accession   NZ_CP053804
Coordinates   285903..286493 (+) Length   196 a.a.
NCBI ID   WP_175229784.1    Uniprot ID   -
Organism   Vibrio cholerae strain SL6Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 280903..291493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY06_RS01315 (HPY06_01320) - 281703..284177 (-) 2475 WP_175229778.1 penicillin-binding protein 1A -
  HPY06_RS01320 (HPY06_01325) pilM 284320..285336 (+) 1017 WP_175229780.1 type IV pilus assembly protein PilM Machinery gene
  HPY06_RS01325 (HPY06_01330) pilN 285326..285910 (+) 585 WP_175229782.1 PilN domain-containing protein Machinery gene
  HPY06_RS01330 (HPY06_01335) pilO 285903..286493 (+) 591 WP_175229784.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY06_RS01335 (HPY06_01340) pilP 286483..287001 (+) 519 WP_175231021.1 pilus assembly protein PilP Machinery gene
  HPY06_RS01340 (HPY06_01345) pilQ 287027..288742 (+) 1716 WP_175229786.1 type IV pilus secretin PilQ Machinery gene
  HPY06_RS01345 (HPY06_01350) aroK 288929..289453 (+) 525 WP_000818616.1 shikimate kinase AroK -
  HPY06_RS01350 (HPY06_01355) aroB 289481..290566 (+) 1086 WP_175229788.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22935.31 Da        Isoelectric Point: 4.3736

>NTDB_id=447544 HPY06_RS01330 WP_175229784.1 285903..286493(+) (pilO) [Vibrio cholerae strain SL6Y]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIELLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=447544 HPY06_RS01330 WP_175229784.1 285903..286493(+) (pilO) [Vibrio cholerae strain SL6Y]
ATGGCTAGTTTGCAAGAGTTAGAATTGGATGAAATCAGTGAATGGCCGCTGCTGCCTCAATTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCGGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGAGCTGCTAAAGCAGGAAG
AGGAAACCGTCAAGGCCACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTATGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCTTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGAAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGGGTAAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTATACCTACCA
GTTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

99.49

100

0.995
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694