Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   O3K_RS10685 Genome accession   NC_018658
Coordinates   2203039..2204049 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli O104:H4 str. 2011C-3493     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2198039..2209049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O3K_RS10655 (O3K_10605) nudB 2199763..2200215 (+) 453 WP_077221229.1 dihydroneopterin triphosphate diphosphatase -
  O3K_RS10660 (O3K_10610) yebC 2200244..2200984 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  O3K_RS10665 (O3K_10615) ruvC 2201019..2201540 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  O3K_RS10670 (O3K_10620) yebB 2201542..2202144 (-) 603 WP_001024930.1 YebB family permuted papain-like enzyme -
  O3K_RS10675 yobI 2202215..2202280 (+) 66 WP_001386853.1 stress response small protein YobI -
  O3K_RS10680 (O3K_10625) ruvA 2202419..2203030 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  O3K_RS10685 (O3K_10630) ruvB 2203039..2204049 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  O3K_RS10690 (O3K_10635) znuB 2204196..2204981 (-) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  O3K_RS10695 (O3K_10640) znuC 2204978..2205733 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  O3K_RS10700 (O3K_10645) znuA 2205812..2206744 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  O3K_RS10705 (O3K_10650) mepM 2206760..2208082 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=44505 O3K_RS10685 WP_000568519.1 2203039..2204049(+) (ruvB) [Escherichia coli O104:H4 str. 2011C-3493]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=44505 O3K_RS10685 WP_000568519.1 2203039..2204049(+) (ruvB) [Escherichia coli O104:H4 str. 2011C-3493]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494

Multiple sequence alignment