Detailed information NTG0444994

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HMY34_RS07160
• Genome accession: NZ_CP053482
• Coordinates: 1455978..1457156 (-)
• Length: 392 a.a.
• NCBI ID: WP_202718573.1
• Uniprot ID: -
• Organism: Thiothrix subterranea strain Ku-5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1450978..1462156

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMY34_RS07140 (HMY34_07135)	argS	1451348..1451917 (+)	570	WP_228288003.1	arginine--tRNA ligase	-
HMY34_RS07145 (HMY34_07140)	-	1451967..1454489 (-)	2523	WP_202718572.1	Ig-like domain-containing protein	-
HMY34_RS07150 (HMY34_07145)	-	1454527..1454964 (-)	438	WP_202715415.1	transposase family protein	-
HMY34_RS07155 (HMY34_07150)	-	1454933..1455433 (-)	501	WP_202715414.1	transposase family protein	-
HMY34_RS07160 (HMY34_07155)	pilU	1455978..1457156 (-)	1179	WP_202718573.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HMY34_RS07165 (HMY34_07160)	-	1457209..1457910 (+)	702	WP_202718574.1	pseudouridine synthase	-
HMY34_RS07170 (HMY34_07165)	-	1457897..1460278 (-)	2382	WP_202718575.1	InlB B-repeat-containing protein	-
HMY34_RS07175 (HMY34_07170)	-	1460311..1461495 (+)	1185	WP_202718576.1	serine protease	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43862.86 Da
• Isoelectric Point: 7.1151

>NTDB_id=444994 HMY34_RS07160 WP_202718573.1 1455978..1457156(-) (pilU) [Thiothrix subterranea strain Ku-5]
MKLKEYLRILAHYDGSDLYLTADLEPKAKFQGKLKAVDKVIMTPEILKAMAYELMNPEQQQQFEKKPEMNLAISEEGIGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTVDGKRVAAIEILLGTPMVRDLIMKGDVHAIKEIMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLNKPRPTAAAPAPEAKPKVADEDFMAKLSLQPKPEVELT

Nucleotide

• Length: 1179 bp

>NTDB_id=444994 HMY34_RS07160 WP_202718573.1 1455978..1457156(-) (pilU) [Thiothrix subterranea strain Ku-5]
ATGAAACTCAAAGAATATCTACGCATCCTCGCCCATTACGACGGTTCCGACCTGTACCTGACCGCCGATCTCGAACCCAA
AGCCAAATTCCAAGGCAAACTCAAAGCCGTCGATAAAGTGATCATGACGCCGGAAATACTCAAAGCGATGGCCTACGAAT
TGATGAACCCTGAACAACAGCAACAGTTTGAAAAGAAACCGGAAATGAACCTTGCCATCAGCGAAGAAGGCATCGGGCGT
TTCCGCGTCAATATATTCAAGCAGCGCCACAAAATCGCAATGGTGATCCGCAATATCAAAACCGACATCCCCAACGCTGA
CAAACTCGGTTTACCGCAAGTCTTGAAAGACGTGATCATGGAAAAACGCGGACTAATCCTCTTCGTCGGCGGCACAGGTT
CGGGCAAATCCACCTCACTCGCCGCCCTGATCGACCACCGCAACGCCAGTGCAGACGGGCATATCATCACCATCGAAGAC
CCTGTGGAATACGTGCATCCCCACAAAAAATGTATCATCAACCAGCGCGAAGTCGGCGTTGACACCGACAGCTACGAAGA
CGCACTCAAAAACACCCTGCGCCAAGCCCCCGACGTGATTTTAATCGGCGAAATTCGCGCCCAAGAAACGATGGAACACG
CCCTAGCCTTCGCTGAAACCGGGCATCTGTGCCTTTCCACCCTGCACGCCAATAACGCCAACCAAGCGCTCGACCGCATT
ATCAATTTCTTCCCAGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATCCCCACCGTGGATGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACACCAATGGTGCGTGACCTGATCA
TGAAAGGCGACGTTCACGCCATCAAAGAAATCATGGAAAAGTCCGAAGAACAGGGGATGCAAACCTTCGACAGCCACCTC
TACAAACTGTATCTGGCTGGGCAAATTTCACTGGCGGAAACCTTGCGCAATGCCGATTCACCTAGCAACTTGAAGCTGAA
GATCAACCTGTCGGGGAATTTGAATAAGCCGCGTCCGACGGCTGCCGCACCTGCGCCAGAGGCAAAGCCCAAGGTGGCAG
ATGAGGATTTCATGGCGAAACTGTCGTTACAGCCGAAGCCGGAAGTGGAGTTGACGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (37-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.769	92.857	0.518
pilU	Acinetobacter baylyi ADP1	54.318	91.582	0.497
pilU	Vibrio cholerae strain A1552	48.997	89.031	0.436
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.623	93.367	0.398