Detailed information NTG0443725

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ITG70_RS02015
• Genome accession: NZ_CP064392
• Coordinates: 432709..433857 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain SRRSH15
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 427709..438857

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG70_RS01990 (ITG70_01990)	-	428872..429168 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
ITG70_RS01995 (ITG70_01995)	-	429165..429758 (-)	594	WP_003084544.1	YggT family protein	-
ITG70_RS02000 (ITG70_02000)	proC	429769..430590 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
ITG70_RS02005 (ITG70_02005)	-	430602..431294 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
ITG70_RS02010 (ITG70_02010)	pilT	431509..432531 (+)	1023	WP_034052146.1	type IV pilus twitching motility protein PilT	Machinery gene
ITG70_RS02015 (ITG70_02015)	pilU	432709..433857 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
ITG70_RS02020 (ITG70_02020)	czcD	433865..434764 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
ITG70_RS02025 (ITG70_02025)	-	434918..435322 (+)	405	WP_003084560.1	NINE protein	-
ITG70_RS02030 (ITG70_02030)	-	435565..436938 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
ITG70_RS02035 (ITG70_02035)	-	436935..438119 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=443725 ITG70_RS02015 WP_003100959.1 432709..433857(+) (pilU) [Pseudomonas aeruginosa strain SRRSH15]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=443725 ITG70_RS02015 WP_003100959.1 432709..433857(+) (pilU) [Pseudomonas aeruginosa strain SRRSH15]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382