Detailed information NTG0443709

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUJ43_RS26025
• Genome accession: NZ_CP064391
• Coordinates: 5662409..5663557 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5657409..5668557

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUJ43_RS26000 (IUJ43_25940)	-	5658560..5658856 (-)	297	WP_031655952.1	DUF167 domain-containing protein	-
IUJ43_RS26005 (IUJ43_25945)	-	5658853..5659446 (-)	594	WP_003084544.1	YggT family protein	-
IUJ43_RS26010 (IUJ43_25950)	proC	5659457..5660278 (-)	822	WP_003114890.1	pyrroline-5-carboxylate reductase	-
IUJ43_RS26015 (IUJ43_25955)	-	5660290..5660982 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IUJ43_RS26020 (IUJ43_25960)	pilT	5661197..5662231 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IUJ43_RS26025 (IUJ43_25965)	pilU	5662409..5663557 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
IUJ43_RS26030 (IUJ43_25970)	czcD	5663565..5664464 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IUJ43_RS26035 (IUJ43_25975)	-	5664618..5665022 (+)	405	WP_003084560.1	NINE protein	-
IUJ43_RS26040 (IUJ43_25980)	-	5665265..5666638 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
IUJ43_RS26045 (IUJ43_25985)	-	5666635..5667819 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=443709 IUJ43_RS26025 WP_003100959.1 5662409..5663557(+) (pilU) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=443709 IUJ43_RS26025 WP_003100959.1 5662409..5663557(+) (pilU) [Pseudomonas aeruginosa strain PartH-Paeruginosa-RM8376]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382