Detailed information NTG0443273

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IUJ44_RS09635
• Genome accession: NZ_CP064375
• Coordinates: 2050116..2051234 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2045116..2056234

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IUJ44_RS09605 (IUJ44_09595)	-	2045945..2046793 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
IUJ44_RS09610 (IUJ44_09600)	-	2047046..2047501 (+)	456	WP_000782976.1	bacteriohemerythrin	-
IUJ44_RS09615 (IUJ44_09605)	-	2047654..2048721 (+)	1068	WP_005144332.1	hypothetical protein	-
IUJ44_RS09620 (IUJ44_09610)	-	2048721..2049044 (+)	324	WP_000442584.1	RnfH family protein	-
IUJ44_RS09625 (IUJ44_09615)	bamE	2049099..2049497 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
IUJ44_RS09630 (IUJ44_09620)	fur	2049609..2050046 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
IUJ44_RS09635 (IUJ44_09625)	pilU	2050116..2051234 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
IUJ44_RS09640 (IUJ44_09630)	pilT	2051262..2052299 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
IUJ44_RS09645 (IUJ44_09635)	-	2052426..2053118 (+)	693	WP_001108520.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=443273 IUJ44_RS09635 WP_000347036.1 2050116..2051234(-) (pilU) [Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=443273 IUJ44_RS09635 WP_000347036.1 2050116..2051234(-) (pilU) [Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392