Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   PMCN06_RS11115 Genome accession   NC_017027
Coordinates   2342688..2344022 (+) Length   444 a.a.
NCBI ID   WP_014668626.1    Uniprot ID   A0A849CKS9
Organism   Pasteurella multocida subsp. multocida str. HN06     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2337688..2349022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMCN06_RS11090 (PMCN06_2233) - 2337764..2340325 (-) 2562 WP_015691137.1 penicillin-binding protein 1A -
  PMCN06_RS11095 (PMCN06_2234) - 2340461..2341234 (+) 774 WP_014668624.1 pilus assembly protein PilM -
  PMCN06_RS11100 (PMCN06_2235) - 2341274..2341789 (+) 516 WP_005751904.1 hypothetical protein -
  PMCN06_RS11105 (PMCN06_2236) - 2341789..2342313 (+) 525 WP_005751903.1 hypothetical protein -
  PMCN06_RS11110 (PMCN06_2237) - 2342316..2342678 (+) 363 WP_014668625.1 pilus assembly protein PilP -
  PMCN06_RS11115 (PMCN06_2238) comE 2342688..2344022 (+) 1335 WP_014668626.1 type IV pilus secretin PilQ Machinery gene
  PMCN06_RS11120 (PMCN06_2239) aroK 2344205..2344732 (+) 528 WP_005717623.1 shikimate kinase AroK -
  PMCN06_RS11125 (PMCN06_2240) aroB 2344749..2345837 (+) 1089 WP_014668627.1 3-dehydroquinate synthase -
  PMCN06_RS11130 (PMCN06_2241) - 2345841..2346746 (+) 906 WP_005723696.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PMCN06_RS11135 (PMCN06_2242) - 2346793..2347098 (-) 306 WP_005723691.1 DUF721 domain-containing protein -
  PMCN06_RS11140 (PMCN06_2243) secM 2347191..2347502 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49267.67 Da        Isoelectric Point: 8.0315

>NTDB_id=44287 PMCN06_RS11115 WP_014668626.1 2342688..2344022(+) (comE) [Pasteurella multocida subsp. multocida str. HN06]
MWRAFRKISFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALQQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IARMKQLELHQEGKSYYLTSPSGVAANDTHHPTSLMTSSIKLHFAKAAEVVKSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFSTNN
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQKTERHQKRELVIFVTPHIIKPSQGSPEQKTTRVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=44287 PMCN06_RS11115 WP_014668626.1 2342688..2344022(+) (comE) [Pasteurella multocida subsp. multocida str. HN06]
ATGTGGCGAGCATTCAGAAAAATATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCGCCTTTAGTCGAAATGTTACAGTATTTAGCATTGCAACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAGAATGAAACAACTTGAGTTACATCAAGAAGGAAAATCCTATTATTTAACTTCCCCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGAGGTGGTGAAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
TAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCAGGAAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTCTCGACCAATAAT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTAGATTATTAGACTTGGAATTAACTGCCTTGGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACAG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTGGAATTTCGAGAAGCTGTATTGGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGAAAACTGAACGTCATCAAAAGCGGGAATTAGTGATTTTTGTCACTCCTCATATTATCAAACCTAGCCA
AGGTTCGCCTGAACAAAAAACAACAAGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A849CKS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

62.037

97.297

0.604
  comE Haemophilus influenzae 86-028NP

61.343

97.297

0.597
  comE Glaesserella parasuis strain SC1401

48.961

97.523

0.477
  pilQ Vibrio campbellii strain DS40M4

39.286

94.595

0.372
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.563

92.793

0.367
  pilQ Vibrio cholerae strain A1552

39.563

92.793

0.367

Multiple sequence alignment