Detailed information NTG0442360

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HCX54_RS14985
• Genome accession: NZ_CP053034
• Coordinates: 3023961..3025079 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain abYQ4
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3018961..3030079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HCX54_RS14975 (HCX54_14955)	-	3022077..3022769 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
HCX54_RS14980 (HCX54_14960)	pilT	3022896..3023933 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HCX54_RS14985 (HCX54_14965)	pilU	3023961..3025079 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HCX54_RS14990 (HCX54_14970)	fur	3025149..3025586 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HCX54_RS14995 (HCX54_14975)	-	3025698..3026096 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HCX54_RS15000 (HCX54_14980)	-	3026151..3026474 (-)	324	WP_000442587.1	RnfH family protein	-
HCX54_RS15005 (HCX54_14985)	-	3026474..3027541 (-)	1068	WP_002019572.1	hypothetical protein	-
HCX54_RS15010 (HCX54_14990)	-	3027694..3028149 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HCX54_RS15015 (HCX54_14995)	-	3028402..3029250 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=442360 HCX54_RS14985 WP_000347039.1 3023961..3025079(+) (pilU) [Acinetobacter baumannii strain abYQ4]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=442360 HCX54_RS14985 WP_000347039.1 3023961..3025079(+) (pilU) [Acinetobacter baumannii strain abYQ4]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395