Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   IRJ28_RS04080 Genome accession   NZ_CP064096
Coordinates   803181..804185 (+) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain N1142-3at     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 798181..809185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ28_RS04050 - 798410..798532 (+) 123 WP_003229707.1 hypothetical protein -
  IRJ28_RS04055 yrzF 798578..799225 (-) 648 WP_003246159.1 phosphotransferase -
  IRJ28_RS04060 iolG 799463..800488 (+) 1026 WP_004398990.1 inositol 2-dehydrogenase -
  IRJ28_RS04065 csbX 800559..801866 (+) 1308 WP_003246197.1 MFS transporter -
  IRJ28_RS04070 bofC 801914..802426 (+) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  IRJ28_RS04075 ruvA 802565..803170 (+) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  IRJ28_RS04080 ruvB 803181..804185 (+) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  IRJ28_RS04085 yrzS 804178..804378 (+) 201 WP_003222669.1 DUF2905 domain-containing protein -
  IRJ28_RS04090 queA 804408..805436 (+) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  IRJ28_RS04095 tgt 805463..806608 (+) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  IRJ28_RS04100 yajC 806645..806911 (+) 267 WP_004398708.1 preprotein translocase subunit YajC -
  IRJ28_RS04105 - 806968..807357 (-) 390 WP_004398667.1 TIGR04086 family membrane protein -
  IRJ28_RS04110 yrbG 807555..808211 (+) 657 WP_004398801.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=442158 IRJ28_RS04080 WP_003229718.1 803181..804185(+) (ruvB) [Bacillus subtilis strain N1142-3at]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=442158 IRJ28_RS04080 WP_003229718.1 803181..804185(+) (ruvB) [Bacillus subtilis strain N1142-3at]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518