Detailed information NTG0441748

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HKJ31_RS06215
• Genome accession: NZ_CP052855
• Coordinates: 1368493..1369623 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain Fillmore
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1363493..1374623

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKJ31_RS06195 (HKJ31_06315)	-	1363817..1364641 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
HKJ31_RS06200 (HKJ31_06320)	-	1365258..1366085 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
HKJ31_RS06205 (HKJ31_06325)	-	1366470..1366682 (-)	213	WP_021358439.1	hypothetical protein	-
HKJ31_RS06210 (HKJ31_06330)	pilT	1367304..1368338 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
HKJ31_RS06215 (HKJ31_06335)	pilU	1368493..1369623 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HKJ31_RS06220 (HKJ31_06340)	-	1370292..1371260 (-)	969	WP_004084952.1	YafY family protein	-
HKJ31_RS06225 (HKJ31_06345)	-	1372043..1372852 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
HKJ31_RS06230 (HKJ31_06350)	hemC	1372906..1373823 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
HKJ31_RS06235 (HKJ31_06355)	-	1373831..1374589 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=441748 HKJ31_RS06215 WP_004084950.1 1368493..1369623(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Fillmore]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=441748 HKJ31_RS06215 WP_004084950.1 1368493..1369623(+) (pilU) [Xylella fastidiosa subsp. multiplex strain Fillmore]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402