Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HKJ32_RS09965 Genome accession   NZ_CP052854
Coordinates   2130336..2131394 (-) Length   352 a.a.
NCBI ID   WP_004086598.1    Uniprot ID   A0A9Q4MGX6
Organism   Xylella fastidiosa subsp. multiplex strain LM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2125336..2136394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKJ32_RS09945 (HKJ32_10150) - 2126567..2128479 (-) 1913 Protein_1966 type IV pilus secretin PilQ -
  HKJ32_RS09950 (HKJ32_10155) - 2128479..2129009 (-) 531 WP_004086595.1 pilus assembly protein PilP -
  HKJ32_RS09955 (HKJ32_10160) - 2129006..2129674 (-) 669 WP_004086596.1 type 4a pilus biogenesis protein PilO -
  HKJ32_RS09960 (HKJ32_10165) - 2129671..2130336 (-) 666 WP_004086597.1 PilN domain-containing protein -
  HKJ32_RS09965 (HKJ32_10170) pilM 2130336..2131394 (-) 1059 WP_004086598.1 type IV pilus biogenesis protein PilM Machinery gene
  HKJ32_RS09970 (HKJ32_10175) - 2131634..2134063 (+) 2430 WP_021358378.1 penicillin-binding protein 1A -
  HKJ32_RS09975 (HKJ32_10180) - 2134367..2135332 (-) 966 WP_004086600.1 aldo/keto reductase -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37539.19 Da        Isoelectric Point: 4.6541

>NTDB_id=441732 HKJ32_RS09965 WP_004086598.1 2130336..2131394(-) (pilM) [Xylella fastidiosa subsp. multiplex strain LM10]
MGLFSKKQSVLVGVDISSTAVKLLQLSRSGNRFKVEHYAVEPLPLNAVAEKGIVEVEQVGEAIRRAVSRSGTKAKFAAAA
VAGSAVITKLIPMPAGLEEQDLEAQIEIEATNYIPYPIEEVSLDFEVLGPVPNNTEMVQVLLAASRSENVELRQSALELG
GLTAKVIDVEALAVENAFSLIAQELSVGSNALVALIDIGATMSTLNVLHSGRSLYAREQLFGGKQLTDEVMHRYGMTYEE
AGQAKRQGGLPQSYDVEVLGPFKDSVIQQISRLLQFFYAGSEYNRVDCIVLAGGCAVIAGLPAMVEERLGVVTVVANPLA
QMTLGAKVQAHTLAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=441732 HKJ32_RS09965 WP_004086598.1 2130336..2131394(-) (pilM) [Xylella fastidiosa subsp. multiplex strain LM10]
GTGGGGCTTTTTTCCAAAAAACAGTCGGTATTGGTTGGTGTCGACATTAGTTCAACTGCAGTGAAGCTCTTACAGCTTTC
CCGCAGTGGTAATCGCTTCAAGGTGGAACACTATGCTGTGGAGCCGCTACCTCTGAATGCAGTCGCCGAGAAGGGCATCG
TTGAAGTCGAGCAGGTTGGTGAAGCAATCCGTCGTGCGGTAAGTCGTTCTGGAACTAAGGCCAAATTTGCCGCTGCTGCG
GTTGCCGGTTCGGCAGTGATCACCAAGTTGATACCGATGCCTGCTGGTCTGGAGGAGCAGGATTTGGAAGCCCAGATAGA
AATAGAGGCTACGAATTACATCCCGTACCCTATTGAAGAGGTCAGCCTAGATTTCGAGGTGCTTGGTCCGGTTCCCAACA
ACACGGAGATGGTCCAAGTTTTGCTGGCTGCGTCTCGTTCGGAGAATGTCGAATTGCGCCAATCTGCGTTGGAGTTGGGT
GGGTTGACTGCCAAGGTTATTGACGTGGAAGCTTTAGCGGTTGAGAACGCTTTCTCTCTTATTGCCCAAGAACTGTCGGT
TGGGAGTAATGCATTGGTTGCATTGATCGACATCGGTGCAACGATGTCGACGCTTAATGTGCTGCATAGTGGTCGCAGTT
TGTACGCGCGCGAGCAGCTATTTGGTGGTAAGCAGCTTACTGACGAAGTGATGCATCGCTATGGGATGACTTACGAGGAG
GCTGGTCAGGCCAAGCGTCAAGGAGGTTTGCCGCAAAGCTATGATGTTGAAGTGCTGGGACCATTTAAGGACTCTGTGAT
ACAGCAGATCAGCCGTTTATTACAGTTTTTCTATGCTGGCAGTGAGTACAACCGAGTTGATTGCATCGTGTTGGCGGGAG
GCTGTGCTGTGATTGCGGGGTTACCGGCTATGGTCGAAGAACGGTTGGGTGTCGTTACCGTTGTGGCTAATCCGCTTGCG
CAGATGACTTTAGGGGCGAAAGTGCAAGCACACACGCTTGCTCAAGATGCACCTGCTTTAATGATTGCTACTGGTCTGGC
TTTGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

48.58

100

0.486
  comM Acinetobacter nosocomialis M2

48.58

100

0.486
  comM Acinetobacter baylyi ADP1

48.011

100

0.48
  pilM Legionella pneumophila strain ERS1305867

45.326

100

0.455