Detailed information NTG0441731

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HKJ32_RS06785
• Genome accession: NZ_CP052854
• Coordinates: 1467196..1468326 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain LM10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1462196..1473326

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HKJ32_RS06765 (HKJ32_06910)	-	1462520..1463344 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
HKJ32_RS06770 (HKJ32_06915)	-	1463961..1464788 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
HKJ32_RS06775 (HKJ32_06920)	-	1465173..1465385 (-)	213	WP_021358439.1	hypothetical protein	-
HKJ32_RS06780 (HKJ32_06925)	pilT	1466007..1467041 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
HKJ32_RS06785 (HKJ32_06930)	pilU	1467196..1468326 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HKJ32_RS06790 (HKJ32_06935)	-	1469020..1469988 (-)	969	WP_004084952.1	YafY family protein	-
HKJ32_RS06795 (HKJ32_06940)	-	1470771..1471580 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
HKJ32_RS06800 (HKJ32_06945)	hemC	1471634..1472551 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
HKJ32_RS06805 (HKJ32_06950)	-	1472559..1473317 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=441731 HKJ32_RS06785 WP_004084950.1 1467196..1468326(+) (pilU) [Xylella fastidiosa subsp. multiplex strain LM10]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=441731 HKJ32_RS06785 WP_004084950.1 1467196..1468326(+) (pilU) [Xylella fastidiosa subsp. multiplex strain LM10]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402