Detailed information NTG0441283

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HIN86_RS15075
• Genome accession: NZ_CP051875
• Coordinates: 3113097..3114215 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain Ab-B004d-c
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3108097..3119215

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIN86_RS15065 (HIN86_15065)	-	3111213..3111905 (-)	693	WP_032000563.1	YggS family pyridoxal phosphate-dependent enzyme	-
HIN86_RS15070 (HIN86_15070)	pilT	3112032..3113069 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
HIN86_RS15075 (HIN86_15075)	pilU	3113097..3114215 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HIN86_RS15080 (HIN86_15080)	fur	3114285..3114722 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
HIN86_RS15085 (HIN86_15085)	-	3114834..3115232 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
HIN86_RS15090 (HIN86_15090)	-	3115287..3115610 (-)	324	WP_032000562.1	RnfH family protein	-
HIN86_RS15095 (HIN86_15095)	-	3115610..3116677 (-)	1068	WP_004748097.1	hypothetical protein	-
HIN86_RS15100 (HIN86_15100)	-	3116830..3117285 (-)	456	WP_000782976.1	bacteriohemerythrin	-
HIN86_RS15105 (HIN86_15105)	-	3117537..3118385 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=441283 HIN86_RS15075 WP_000347036.1 3113097..3114215(+) (pilU) [Acinetobacter baumannii strain Ab-B004d-c]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=441283 HIN86_RS15075 WP_000347036.1 3113097..3114215(+) (pilU) [Acinetobacter baumannii strain Ab-B004d-c]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACCGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAGCAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392