Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   IQS14_RS00495 Genome accession   NZ_CP063492
Coordinates   75371..76609 (-) Length   412 a.a.
NCBI ID   WP_203154676.1    Uniprot ID   -
Organism   Escherichia coli strain EF5-18-41     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 70371..81609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS14_RS00475 (IQS14_00475) dam 70983..71819 (-) 837 WP_000742137.1 adenine-specific DNA-methyltransferase -
  IQS14_RS00480 (IQS14_00480) damX 71926..73212 (-) 1287 WP_000343222.1 cell division protein DamX -
  IQS14_RS00485 (IQS14_00485) aroB 73304..74392 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  IQS14_RS00490 (IQS14_00490) aroK 74449..74970 (-) 522 WP_000818618.1 shikimate kinase AroK -
  IQS14_RS00495 (IQS14_00495) comE 75371..76609 (-) 1239 WP_203154676.1 DNA uptake porin HofQ Machinery gene
  IQS14_RS00500 (IQS14_00500) hofP 76521..76925 (-) 405 WP_001322941.1 DNA utilization protein HofP -
  IQS14_RS00505 (IQS14_00505) hofO 76915..77355 (-) 441 WP_000367364.1 DNA utilization protein HofO -
  IQS14_RS00510 (IQS14_00510) hofN 77339..77878 (-) 540 WP_001069323.1 DNA utilization protein HofN -
  IQS14_RS00515 (IQS14_00515) hofM 77878..78657 (-) 780 WP_001377090.1 DNA utilization protein HofM -
  IQS14_RS00520 (IQS14_00520) mrcA 78777..81329 (+) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44786.37 Da        Isoelectric Point: 6.1846

>NTDB_id=440105 IQS14_RS00495 WP_203154676.1 75371..76609(-) (comE) [Escherichia coli strain EF5-18-41]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVRGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KAALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATLVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=440105 IQS14_RS00495 WP_203154676.1 75371..76609(-) (comE) [Escherichia coli strain EF5-18-41]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACACTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGAGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGGCAGGAAGGCAATATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAGCGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
TGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

36.927

100

0.391
  pilQ Vibrio campbellii strain DS40M4

38.005

100

0.388
  comE Haemophilus influenzae 86-028NP

36.697

100

0.388
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.952

100

0.367