Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HHJ60_RS07820 Genome accession   NZ_CP051638
Coordinates   1673403..1673834 (-) Length   143 a.a.
NCBI ID   WP_035687024.1    Uniprot ID   A0A0F5ESJ8
Organism   Avibacterium paragallinarum strain ADL-AP15     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1668403..1678834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ60_RS07800 (HHJ60_07800) ispC 1669042..1670238 (-) 1197 WP_035687018.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
  HHJ60_RS07805 (HHJ60_07805) frr 1670418..1670975 (-) 558 WP_017807054.1 ribosome recycling factor -
  HHJ60_RS07810 (HHJ60_07810) pyrH 1671000..1671716 (-) 717 WP_035687020.1 UMP kinase -
  HHJ60_RS07815 (HHJ60_07815) pilB 1671966..1673390 (-) 1425 WP_035687022.1 GspE/PulE family protein Machinery gene
  HHJ60_RS07820 (HHJ60_07820) pilA 1673403..1673834 (-) 432 WP_035687024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HHJ60_RS07825 (HHJ60_07825) ampD 1673974..1674528 (+) 555 WP_035687059.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HHJ60_RS07830 (HHJ60_07830) - 1674627..1675046 (+) 420 WP_124994872.1 hypothetical protein -
  HHJ60_RS07835 (HHJ60_07835) rppH 1675618..1676211 (+) 594 WP_035687029.1 RNA pyrophosphohydrolase -
  HHJ60_RS07840 (HHJ60_07840) - 1676214..1677005 (+) 792 WP_035687031.1 sulfite exporter TauE/SafE family protein -
  HHJ60_RS07845 (HHJ60_07845) lgt 1677015..1677815 (+) 801 WP_035687035.1 prolipoprotein diacylglyceryl transferase -
  HHJ60_RS07850 (HHJ60_07850) - 1677827..1678678 (+) 852 WP_017807045.1 thymidylate synthase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15205.70 Da        Isoelectric Point: 9.8115

>NTDB_id=440065 HHJ60_RS07820 WP_035687024.1 1673403..1673834(-) (pilA) [Avibacterium paragallinarum strain ADL-AP15]
MQNPTHLKIKKGFTLIELMIVIAIVAILATIAIPAYQNYTKKAAISELIQAASPYRSEVELCIYNTGATTKCNAGSNGIQ
KKLTNKGKVASIDVQKGTITVKGKDNLSDITYSLKASGDASKGVSWTVNCGKSVEFFPAGFCG

Nucleotide


Download         Length: 432 bp        

>NTDB_id=440065 HHJ60_RS07820 WP_035687024.1 1673403..1673834(-) (pilA) [Avibacterium paragallinarum strain ADL-AP15]
ATGCAAAACCCAACCCATTTAAAAATCAAAAAAGGCTTTACTTTGATTGAACTGATGATCGTGATTGCGATTGTGGCGAT
TTTAGCGACCATTGCGATTCCTGCCTATCAAAATTACACCAAGAAAGCGGCAATATCTGAGTTAATTCAAGCCGCAAGCC
CCTATCGTTCAGAGGTGGAATTATGTATTTATAATACGGGGGCTACCACGAAATGTAATGCGGGTTCAAATGGAATTCAG
AAAAAATTAACGAATAAAGGGAAAGTGGCTTCTATTGATGTACAAAAAGGCACGATTACGGTTAAAGGAAAAGATAATCT
TTCTGATATTACTTATTCATTAAAAGCCAGTGGAGATGCCTCAAAAGGTGTATCTTGGACGGTAAATTGTGGAAAATCTG
TAGAGTTTTTCCCAGCGGGATTTTGCGGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5ESJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.31

100

0.601
  pilA Haemophilus influenzae 86-028NP

57.931

100

0.587
  pilA Glaesserella parasuis strain SC1401

46.809

98.601

0.462
  pilA Vibrio campbellii strain DS40M4

34.615

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

37.324

99.301

0.371