Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   IQS25_RS16480 Genome accession   NZ_CP063467
Coordinates   3432703..3433371 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EM20-18-50     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3427703..3438371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IQS25_RS16450 (IQS25_16460) yicC 3427722..3428585 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  IQS25_RS16455 (IQS25_16465) rph 3428712..3429428 (+) 717 WP_001247089.1 ribonuclease PH -
  IQS25_RS16460 (IQS25_16470) pyrE 3429494..3430135 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  IQS25_RS16465 (IQS25_16475) slmA 3430172..3430768 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  IQS25_RS16470 (IQS25_16480) dut 3430875..3431333 (-) 459 WP_000976070.1 dUTP diphosphatase -
  IQS25_RS16475 (IQS25_16485) coaBC 3431311..3432531 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  IQS25_RS16480 (IQS25_16490) radC 3432703..3433371 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  IQS25_RS16485 (IQS25_16495) rpmB 3433588..3433824 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  IQS25_RS16490 (IQS25_16500) rpmG 3433845..3434012 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  IQS25_RS16495 (IQS25_16505) mutM 3434110..3434919 (+) 810 WP_203139528.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  IQS25_RS16500 (IQS25_16510) coaD 3434958..3435437 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  IQS25_RS16505 (IQS25_16515) waaA 3435445..3436722 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  IQS25_RS16510 (IQS25_16520) rfaQ 3437135..3438193 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=439677 IQS25_RS16480 WP_001297375.1 3432703..3433371(+) (radC) [Escherichia coli strain EM20-18-50]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=439677 IQS25_RS16480 WP_001297375.1 3432703..3433371(+) (radC) [Escherichia coli strain EM20-18-50]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGTTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496