Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IPV48_RS28360 Genome accession   NZ_CP063386
Coordinates   6092282..6092905 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain ST1076_d118limb1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6087282..6097905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IPV48_RS28340 (IPV48_28320) aroB 6087877..6088983 (-) 1107 WP_003112148.1 3-dehydroquinate synthase -
  IPV48_RS28345 (IPV48_28325) aroK 6089033..6089551 (-) 519 WP_003095833.1 shikimate kinase AroK -
  IPV48_RS28350 (IPV48_28330) pilQ 6089563..6091707 (-) 2145 WP_031686163.1 type 4a pilus secretin PilQ Machinery gene
  IPV48_RS28355 (IPV48_28335) comL/pilP 6091761..6092285 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  IPV48_RS28360 (IPV48_28340) pilO 6092282..6092905 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  IPV48_RS28365 (IPV48_28345) pilN 6092902..6093498 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  IPV48_RS28370 (IPV48_28350) pilM 6093498..6094562 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  IPV48_RS28375 (IPV48_28355) - 6094747..6097218 (+) 2472 WP_031686164.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=438355 IPV48_RS28360 WP_003103268.1 6092282..6092905(-) (pilO) [Pseudomonas aeruginosa strain ST1076_d118limb1]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=438355 IPV48_RS28360 WP_003103268.1 6092282..6092905(-) (pilO) [Pseudomonas aeruginosa strain ST1076_d118limb1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401