Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HF888_RS08115 Genome accession   NZ_CP051183
Coordinates   1775527..1776672 (+) Length   381 a.a.
NCBI ID   WP_007017831.1    Uniprot ID   Q1N4I1
Organism   Bermanella marisrubri strain RED65     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1770527..1781672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF888_RS08085 (HF888_08085) - 1771425..1771658 (+) 234 WP_040297696.1 DUF1289 domain-containing protein -
  HF888_RS08090 (HF888_08090) - 1771655..1772320 (-) 666 WP_007017826.1 NAD(P)H-binding protein -
  HF888_RS08095 (HF888_08095) - 1772320..1772763 (-) 444 WP_007017827.1 TerB family tellurite resistance protein -
  HF888_RS08100 (HF888_08100) tal 1772767..1773729 (-) 963 WP_007017828.1 transaldolase -
  HF888_RS08105 (HF888_08105) dusA 1773880..1774872 (-) 993 WP_007017829.1 tRNA dihydrouridine(20/20a) synthase DusA -
  HF888_RS08110 (HF888_08110) - 1775075..1775326 (+) 252 WP_007017830.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  HF888_RS08115 (HF888_08115) pilU 1775527..1776672 (+) 1146 WP_007017831.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HF888_RS08120 (HF888_08120) - 1776800..1777558 (+) 759 WP_007017832.1 hypothetical protein -
  HF888_RS08125 (HF888_08125) - 1777768..1778628 (+) 861 WP_007017833.1 CZB domain-containing protein -
  HF888_RS08130 (HF888_08130) gorA 1778663..1780006 (-) 1344 WP_007017834.1 glutathione-disulfide reductase -
  HF888_RS08135 (HF888_08135) - 1780021..1780551 (-) 531 WP_007017835.1 type II secretion system protein M -
  HF888_RS08140 (HF888_08140) gspL 1780544..1781644 (-) 1101 WP_007017836.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42143.23 Da        Isoelectric Point: 6.5120

>NTDB_id=436699 HF888_RS08115 WP_007017831.1 1775527..1776672(+) (pilU) [Bermanella marisrubri strain RED65]
MSLIKYLQVLAKHDGSDLYLSTGAVPSAKFQGTLKQLGKEPLKSGQIAKMIESVMDEDQKVEFEEELELNMAVSIPSGGR
FRLNMFKQRNEVSVVARNIVAEIPEFEKLGLPEVLKEVVMAKRGLILFVGGTGSGKSTSLASLIDHRNRHSAGHIITIED
PIEFVHKHRKSIINQREVGVDTKSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLQDLGLNMQAIISQRLIPTVDKKRVAAVEVLLGNTTIQDLVMKGDTSGIKEIMAKSENLGMQTFDAAL
FKLHKAGKITMNEALKNADSENNLRLRIKLDSGEGANEKNAGGFSLENDHDEQTEQELQSA

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=436699 HF888_RS08115 WP_007017831.1 1775527..1776672(+) (pilU) [Bermanella marisrubri strain RED65]
ATGTCCCTCATTAAATATCTTCAAGTATTAGCAAAGCACGATGGATCGGATTTGTATTTAAGCACGGGCGCAGTTCCAAG
CGCCAAATTCCAAGGAACATTAAAACAATTAGGTAAAGAGCCCTTAAAGTCTGGACAGATTGCTAAGATGATCGAATCCG
TAATGGATGAAGATCAGAAGGTTGAGTTTGAGGAAGAGCTAGAGCTTAACATGGCAGTTAGTATTCCTAGTGGTGGTCGC
TTCCGGTTGAATATGTTTAAACAAAGAAATGAAGTGTCCGTTGTAGCGAGAAACATCGTTGCCGAAATCCCCGAATTTGA
AAAGCTTGGCTTGCCAGAAGTTCTAAAGGAAGTGGTCATGGCAAAGCGCGGATTGATTTTGTTTGTTGGTGGTACAGGCT
CAGGTAAATCCACTTCGCTTGCGTCACTGATTGATCACAGGAATCGCCACAGTGCCGGTCACATTATTACTATCGAAGAC
CCGATAGAGTTTGTTCACAAACACAGGAAGTCTATCATTAATCAAAGGGAGGTTGGTGTAGATACAAAGAGCTTCCATGC
TGCTTTAAAAAATACGTTGCGACAAGCTCCTGATGTGATTTTGATTGGAGAAATTCGCGATCGCGAGACAATGGAGCATG
CATTAGCATTCGCTGAAACTGGGCATTTGGCGATATCTACGCTGCATGCGAATAATGCGAACCAAGCACTCGACCGCATC
GTAAACTTCTTTCCCGAAGAAAGGCGTCCGCAGTTACTTCAAGATTTGGGTTTGAATATGCAAGCTATTATTTCACAGCG
TTTGATACCAACCGTTGACAAAAAACGCGTTGCAGCCGTAGAAGTCTTGTTGGGGAATACAACGATTCAAGACTTGGTAA
TGAAAGGTGATACGAGTGGAATAAAAGAAATTATGGCTAAATCAGAAAACCTAGGGATGCAAACATTTGATGCGGCGTTA
TTCAAGCTACATAAAGCTGGAAAGATTACAATGAATGAGGCATTAAAGAACGCAGATTCCGAAAACAATCTAAGACTGCG
TATTAAGTTAGACAGTGGTGAAGGTGCTAATGAGAAGAACGCCGGAGGGTTTAGCTTAGAAAACGATCATGATGAACAAA
CCGAGCAAGAGTTACAGAGTGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1N4I1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

54.444

94.488

0.514
  pilU Pseudomonas stutzeri DSM 10701

54.108

92.651

0.501
  pilU Vibrio cholerae strain A1552

48.78

96.85

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.575

93.963

0.409