Detailed information NTG0436285

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IL983_RS27250
• Genome accession: NZ_CP063047
• Coordinates: 5922509..5923657 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain KC-Tt-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5917509..5928657

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IL983_RS27225 (IL983_27225)	-	5918660..5918956 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IL983_RS27230 (IL983_27230)	-	5918953..5919546 (-)	594	WP_003084544.1	YggT family protein	-
IL983_RS27235 (IL983_27235)	proC	5919557..5920378 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
IL983_RS27240 (IL983_27240)	-	5920390..5921082 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IL983_RS27245 (IL983_27245)	pilT	5921297..5922331 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IL983_RS27250 (IL983_27250)	pilU	5922509..5923657 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
IL983_RS27255 (IL983_27255)	czcD	5923665..5924564 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IL983_RS27260 (IL983_27260)	-	5924719..5925123 (+)	405	WP_003084560.1	NINE protein	-
IL983_RS27265 (IL983_27265)	-	5925366..5926739 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
IL983_RS27270 (IL983_27270)	-	5926736..5927920 (+)	1185	WP_197599397.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=436285 IL983_RS27250 WP_003110016.1 5922509..5923657(+) (pilU) [Pseudomonas aeruginosa strain KC-Tt-1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=436285 IL983_RS27250 WP_003110016.1 5922509..5923657(+) (pilU) [Pseudomonas aeruginosa strain KC-Tt-1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATACCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382